Tumor Transplantation
Tumor Transplantation - Related Videos
03:59
Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models
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2023
The video explains a stepwise surgical method for orthotopic implantation of a tumor fragment from a cancerous donor to the pancreas of a non-cancerous recipient mice for development of pancreatic cancer. Orthotopically implanted PDAC could recapture the tissue microenvironment of human pancreatic cancers than conventional subcutaneous (SC) models more accurately.
09:43
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
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Cited by 22 •
2017
The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
08:01
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
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Cited by 35 •
2011
Limiting dilution cell transplantation assays are used to determine the frequency of tumor-propagating cells. This protocol describes a method for generating syngeneic zebrafish that develop fluorescently-labeled leukemia and details how to isolate and transplant these leukemia cells at limiting dilution into the peritoneal cavity of adult zebrafish.
04:17
Long-term Orthotopic Transplantation of Tumor Cells into Zebrafish Embryos: An Efficient Method to Deliver Tumor Cells into the Fourth Ventricle of Zebrafish Embryos for Long-term Analysis of Tumor Cell Behavior
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2025
This video demonstrates the orthotopic transplantation of zebrafish brain tumor cells into the fourth ventricle of an immunocompetent zebrafish embryo. This procedure allows for the long-term study of tumor cell invasion and behavior.
04:15
Bladder Tumor Organoid Model: A Method for Orthotopic Transplantation of Bladder Organoids into Recipient Murine Bladder
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2023
In this video, we describe the orthotopic transplantation of cultured bladder tumor organoids into the murine bladder to study the role of the organ environment on developing tumors.
FAQs
Compatibility between the tumor donor and recipient affects whether transplanted material can survive and establish an expanding tumor. Syngeneic models use compatible subjects, whereas allogeneic models involve different genetic backgrounds, making host recognition an important variable. These distinctions help investigators separate tumor-intrinsic behavior from effects caused by immune compatibility and the recipient’s biological response.
Successful engraftment requires transplanted tumor cells or tissue to persist within the recipient and interact with surrounding host tissues. The tumor microenvironment, meaning the local tissue setting around the tumor, can influence growth and progression. Studying this interaction allows researchers to examine how cancer behavior depends not only on the transplanted material but also on conditions supplied by the host.
These models emphasize different relationships between tumor and host. Syngeneic systems provide donor-recipient compatibility, allogeneic systems introduce differences between them, and xenografts place tumor material in a different biological host context. Comparing these approaches helps researchers investigate tumor progression, host responses, metastasis, and the effects of immune activity under distinct experimental conditions.
At a high level, the experiment requires transferring tumor cells or tissue into a living recipient, then determining whether the material survives and engrafts. Researchers subsequently examine its interaction with host tissues and monitor outcomes such as tumor growth or progression. The selected donor-recipient relationship and the recipient’s immune activity are central conditions throughout this workflow.
Tumor transplantation models are used to investigate how cancers grow, progress, and interact with host tissues. They also provide experimental systems for examining metastasis and testing anticancer drugs or immunotherapies before clinical evaluation. Because different model types emphasize compatibility, immune activity, or host context, researchers can select an approach suited to the biological question being studied.
Results can reveal how tumors respond to treatment and how cancer interacts with host biology, but they do not necessarily predict patient outcomes directly. Experimental hosts may differ from patients in compatibility, immune activity, and tissue environment. Consequently, tumor transplantation supports preclinical assessment while requiring caution when translating observed drug or immunotherapy effects to clinical care.