Ultra Low Attachment Plates
Ultra Low Attachment Plates - Related Videos
11:24
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
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Cited by 9 •
2019
A protocol to create gene modified human pseudoislets from dispersed human islet cells that are transduced by lentivirus carrying short hairpin RNA (shRNA) is presented. This protocol utilizes readily available enzyme and culture vessels, can be performed easily, and produces genetically modified human pseudoislets suitable for functional and morphological studies.
01:20
Attachment
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2025
Attachment is vital for infant development, as warm social interactions support growth and well-being. In a classic 1958 study by Harry Harlow, the significance of warmth and comfort in forming attachments was examined. Harlow separated newborn monkeys from their mothers and provided two artificial "mothers": one made of cold wire and the other covered in soft cloth. Despite the wire mother offering food, the infant monkeys preferred the comfort of the cloth mother, demonstrating that physical...
02:14
Microscopy-Based Evaluation of Bacterial Attachment to Plant Root Surfaces
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2026
Source: Matthysse, A. G. Adherence of Bacteria to Plant Surfaces Measured in the Laboratory. J. Vis. Exp. (2018).This video demonstrates the evaluation of bacterial attachment to plant roots using phase-contrast microscopy. Root samples from seedlings incubated with bacteria are first mounted to check for free bacteria. The samples are then washed to remove loosely attached bacteria and remounted using a suitable coverslip to prevent damage. Finally, the number of bacteria attached to the root...
06:13
Solid Plate-based Dietary Restriction in Caenorhabditis elegans
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Cited by 19 •
2011
Here we present a protocol for performing solid plate-based dietary restriction in C. elegans with killed bacteria.
04:58
Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue
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2025
This video demonstrates a vibratome-based procedure to generate viable, ultra-thin sections of pig heart. The prepared tissue slices can be cultured and used as model systems for pharmacological testing.
FAQs
Their non-adhesive surface limits protein-mediated attachment and cell spreading. Instead of flattening across the vessel, cells remain suspended, allowing cell-cell interactions to produce clusters or other three-dimensional assemblies. This surface behavior makes the plates useful when researchers need to examine cells without the attachment-dependent organization typical of conventional culture vessels.
When cells are maintained under suitable culture conditions, reduced surface adhesion allows them to cluster with one another rather than spread on the plate. The resulting spheroids or aggregates can develop more consistent sizes and shapes, which supports clearer comparisons of cell viability, differentiation, signaling, and responses across experimental samples.
Three-dimensional assemblies provide a culture context that can better represent cell behavior than a conventional monolayer. Their organization enables researchers to investigate outcomes such as viability, differentiation, signaling, and treatment responses in a model that reflects cellular arrangement in three dimensions, rather than relying only on surface-spread cells.
Cells are introduced into the engineered vessels and maintained under culture conditions that support suspension and aggregation. During culture, researchers can monitor whether cells remain suspended and form spheroids or other assemblies, then evaluate features such as aggregate size and shape alongside biological outcomes relevant to the experiment.
The plates support several model systems, including stem cells, organoids, tumor spheroids, and general cell aggregation experiments. Researchers select them when maintaining cells in suspension or generating three-dimensional assemblies is important for the study, particularly when the experimental question concerns organization, differentiation, signaling, or treatment behavior.
Researchers can use spheroids and other aggregates as three-dimensional models for assessing treatment responses. More standardized aggregate size and shape improve the consistency of comparisons between samples, while measurements of viability, differentiation, and signaling help characterize how cells respond to experimental treatments within the model.