Vortex Emulsification

Vortex emulsification is a mixing technique that uses vortex motion to disperse one immiscible liquid within another, producing an emulsion of small droplets for biological and chemical work. As a tube or vessel rotates rapidly, turbulence and shear forces break the dispersed phase into droplets, while surfactants or other stabilizers can reduce interfacial tension and slow droplet coalescence. In biology, the method supports sample homogenization, reagent preparation, cell and biomolecule workflows, and controlled emulsion formation for assays or encapsulation. Droplet size and stability depend on mixing speed, time, liquid properties, and formulation, making process control important for reproducible results.

Vortex Emulsification - Related Videos

Research

JoVE Journal - Environment
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Implementation of a Hyperbolic Vortex Plasma Reactor for the Removal of Micropollutants in Water

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2025

This study presents the methodology for the generation of six different types of plasma discharges within a Hyperbolic Vortex Plasma Reactor for the degradation of micropollutants in water, including pharmaceuticals and per- and polyfluoroalkyl substances (PFAS).

Research

JoVE Journal - Biology
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Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

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Cited by 1 •

2021

Water-in-oil droplet assays are useful for analytical chemistry, enzyme evolution, and single cell analysis, but typically require microfluidics to form the droplets. Here, we describe particle templated emulsification, a microfluidic-free approach to perform droplet assays.

Research

JoVE Journal - Bioengineering

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Cited by 2 •

2014

A microfluidic vortex assisted electroporation platform was developed for sequential delivery of multiple molecules into identical cell populations with precise and independent dosage control. The system’s size based target cell purification step preceding electroporation aided to enhance molecular delivery efficiency and processed cell viability.

Education

JoVE Science Education - Engineering

Flow Visualization in a Water Tunnel: Observing the Leading-edge Vortex Over a Delta Wing

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2023

Source: Jose Roberto Moreto, Gustaaf Jacobs and Xiaofeng Liu, Department of Aerospace Engineering, San Diego State University, San Diego, California The delta wing, shown in Figure 1D, is a popular design in high-speed airplanes due to its superb performance in transonic and supersonic flight regimes. This type of wing has a small aspect ratio and high sweep angle, which reduces drag at high subsonic, transonic and supersonic flight regimes. The aspect ratio is defined as the wing span divided...

Scanning SQUID Study of Vortex Manipulation by Local Contact

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Cited by 1 •

2017

We present a protocol for manipulation of individual vortices in thin superconducting films, using local mechanical contact. The method does not include applying current, magnetic field or additional fabrication steps.

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