Western Diet Mice
Western Diet Mice - Related Videos
09:10
Palatable Western-style Cafeteria Diet as a Reliable Method for Modeling Diet-induced Obesity in Rodents
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Cited by 42 •
2019
This protocol describes the use of a highly palatable, western-style cafeteria diet to model overeating and obesity in rodents. Here, we provide a detailed outline of food selection, preparation and measurement, and explain methodological factors that assist in generating a robust and reproducible phenotype.
11:43
The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
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Cited by 30 •
2014
This protocol explores the latest advancements in performing Western blot analyses. These novel modifications employ a Bis-Tris gel system with a 35 min electrophoresis run time, a 7 min dry blotting transfer system, and infrared fluorescent protein detection and imaging that generates higher resolution, quality, sensitivity, and improved accuracy of Western data.
08:48
The Western Blot
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2023
Western Blotting is used to identify the presence of specific proteins in electrophoretically separated samples. Following separation by a technique known as sodium dodecyl sulfate polyacrylamide gel electrophoresis, or SDS-PAGE, western transfer is used to move proteins from a polyacrylamide gel onto a piece of membrane which traps the proteins in their respective locations. Next, the membranes are probed with antibodies in a process called immunboblotting. Immunoblotting uses...
07:27
The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury
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Cited by 14 •
2017
Here we describe a common method to induce chronic liver injury in mice by feeding of a choline-deficient and ethionine-supplemented (CDE) diet. We demonstrate health monitoring, liver perfusion, isolation, and preservation. A time course of six weeks can inform about liver injury, pathohistology, fibrosis, inflammatory, and liver progenitor cell responses.
07:45
Western Blotting: Sample Preparation to Detection
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Cited by 49 •
2010
Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract.
FAQs
In Western diet mice, excess dietary fat and sugar can increase energy intake relative to expenditure, shifting energy balance toward weight gain. Because the diet is also often energy-dense, its effects reflect both nutrient composition and total energy load. This makes the model useful for examining how diet-driven changes contribute to the development of metabolic disease.
Glucose handling, lipid metabolism, and inflammatory responses provide complementary evidence of diet-related disruption. Glucose measurements indicate how effectively the organism regulates blood sugar, lipid-related measures reflect altered fat processing, and inflammatory responses reveal changes associated with the dietary challenge. Considering these domains together helps biologists connect energy imbalance with broader metabolic and disease-related outcomes.
Energy balance matters because it connects the diet's composition to its observable consequences. Excess fat and sugar can promote weight gain, while the resulting metabolic changes may include impaired glucose regulation, altered lipid metabolism, and inflammation. Researchers therefore interpret disease-related findings as effects of a linked nutritional and physiological process rather than an isolated outcome.
Researchers establish Western diet mice by feeding laboratory mice the specified high-fat, high-sugar, energy-dense diet, then examining the resulting biological changes. The study can focus on glucose handling, lipid metabolism, inflammatory responses, weight gain, or disease-related outcomes. This workflow allows investigators to connect a controlled dietary exposure with measurable changes relevant to the research question.
The model can be used to test how nutrition, genetic factors, drugs, or lifestyle interventions influence disease development. Investigators can examine whether a selected intervention changes weight gain, metabolic regulation, inflammation, or other diet-associated outcomes. This makes the system useful for comparing potential prevention or treatment strategies within a controlled biological research setting.
Western diet mice provide a biological setting for studying obesity, insulin resistance, cardiovascular risk, and other diet-related disease processes. Findings can help clarify how dietary patterns connect to metabolic and inflammatory changes, while intervention studies can reveal factors that modify those effects. This context supports research aimed at understanding prevention and treatment of diet-associated disease.