White-eyed Flies

White-eyed flies are fruit flies, typically Drosophila melanogaster, that lack the red eye pigmentation seen in wild-type individuals and serve as a classic model for studying heredity. The phenotype usually results from altered function of the white gene, which encodes a transporter needed to move pigment precursors into developing eye cells; without this transport, ommochrome and pteridine pigments do not accumulate normally. Researchers use white-eyed flies to investigate mutation, gene function, sex-linked inheritance, and genetic crosses. Their readily scored eye color also makes them valuable for teaching Mendelian principles and analyzing how specific genes produce observable traits.

White-eyed Flies - Related Videos

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JoVE EoE - Drosophila melanogaster (fruit fly)

Dissection of the Eye-Brain Complex from Fly Pupae: A Method to Isolate Retinal Tissue

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2023

This video describes how to dissect and isolate the eye-brain complex from Drosophila pupae. The featured protocol demonstrates the procedure yielding high-quality tissue, compatible with, for example, immunostaining, as well as, other gene expression experiments.

The FlyBar: Administering Alcohol to Flies

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Cited by 15 •

2014

Drosophila has emerged as a significant model system for dissecting the cellular and molecular underpinnings of behavioral responses to alcohol. Here we present a protocol for the collection of alcohol sensitivity data in a circadian context that can be easily applied to other experiments and is well-suited for undergraduate research.

A Single-fly Assay for Foraging Behavior in Drosophila

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Cited by 12 •

2013

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

Drosophila Adult Brain Dissection: A Method in Fly Neurobiology

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2023

This video describes how to dissect and isolate the adult Drosophila brain–a procedure necessary for visualizing the organ, which is otherwise obscured by the cuticle, eye, and tracheal tissue. The example protocol shows a detailed demonstration yielding high-quality preparations that can be used for immunostaining and imaging methods in Drosophila neurobiology.

Infecting Flies with Bacterial Suspension Using a Nanoinjector

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2025

This video demonstrates the nano-injection of bacterial suspension into the fly body cavity, resulting in bacterial multiplication and infection. The fly's immune response clears some bacteria, while others escape immune clearance and survive within the host causing an infection.

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