Whole Cell Method
Whole Cell Method - Related Videos
06:11
An Efficient Method to Obtain Dedifferentiated Fat Cells
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Cited by 4 •
2016
We have modified the conditions for DFAT cell generation and provide herein information regarding the use of an improved growth medium for the production of these cells.
09:57
A Versatile Method of Patterning Proteins and Cells
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Cited by 10 •
2017
This report describes a simple, easy to perform technique, using low pressure vacuum, to fill microfluidic channels with cells and substrates for biological research.
03:45
Tracheotomy: A Method for Transplantation of Stem Cells to the Lung
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Cited by 5 •
2007
Significant breakthroughs in stem cell identification are continuously being made. To translate these discoveries, however, novel methods for cellular delivery must be devised. Here I report that the airways provide a safe route for stem cell transplantation to the lungs.
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
09:18
A Sensitive Method to Quantify Senescent Cancer Cells
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Cited by 39 •
2013
Whether senescence prevents or promotes tumorigenesis remains controversial. Since chemotherapeutical drugs can induce cancer cells to senesce, studying senescence is essential for proposing new therapies. However, the standard and broadly used β-galactosidase assay presents major drawbacks. We propose here a rapid and sensitive flow cytometry-based assay to quantify senescence.
FAQs
Its value comes from retaining communication among membranes, organelles, and molecular pathways while a cell responds to a defined stimulus. Because these systems remain coordinated, measurements can connect a molecular change with a cellular outcome such as altered metabolism, transport, electrical activity, growth, or gene expression. This makes the approach useful for studying physiology and signaling in context.
Compared with assays of purified components, the Whole Cell Method can reveal effects that depend on interactions between multiple cellular systems. An isolated membrane protein or molecular pathway may behave differently outside the cell’s regulatory environment. Measuring the intact response therefore helps determine whether a mechanism produces a meaningful change in cell behavior.
Cell type and experimental conditions strongly influence outcomes. The same stimulus can produce different results in different cells because cellular organization and baseline activity vary. Researchers therefore interpret growth, metabolism, transport, electrical activity, or gene expression in relation to the specific cells and conditions tested. This is essential when comparing experiments or extending findings to another biological context.
Readouts should be matched to the biological question. Measurements of growth or metabolism describe broader functional changes, whereas transport and electrical activity can indicate effects on cellular operations. Changes in gene expression provide another view of how cells respond to a stimulus. Considering several outcomes together can help link a molecular pathway to integrated cellular behavior rather than relying on one measurement alone.
Researchers typically keep the cells intact and living, expose them to a defined condition or stimulus, and then measure a selected response. The measured endpoint may be growth, metabolism, transport, electrical activity, or gene expression. Experimental interpretation should record the cell type and conditions because those factors shape the result. This workflow connects the imposed change with the cell’s resulting behavior.
The method is useful when the research question concerns physiology, signaling, disease processes, pharmacology, or biotechnology. In each setting, the goal is to observe how a stimulus or defined condition affects cellular behavior through coordinated pathways. Its findings can provide context that purified-component studies lack, while dependence on cell type and conditions must remain part of the interpretation.