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Chapters
- 00:05Title
- 02:19General Membrane Labeling with PKH26 Cell Tracking Dye
- 05:43Flow Cytometer Setup and Staining Verification
- 07:34Proliferation Model Selection for Cell Division Monitoring by Dye Dilution
- 10:16Results: Optimization of Cell Tracking Dye Staining for Monitoring Cell Division
- 22:03Conclusion
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
Tags
Optimized StainingProliferation ModelingCell Division MonitoringCell Tracking DyesFlow CytometryImage CytometryStem CellsProgenitor CellsMembrane TransferPrecursor Cell ProliferationImmune Regulatory CellsEffector Cell FunctionCommercially Available DyesMembrane DyesProtein DyesMulticolor StudiesOptimal Use Of Dyes
