Wire Myography System
Wire Myography System - Related Videos
07:51
Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
0 Views •
Cited by 37 •
2011
An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.
09:42
Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
0 Views •
Cited by 16 •
2018
The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.
07:25
Assessing Murine Resistance Artery Function Using Pressure Myography
0 Views •
Cited by 18 •
2013
In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
09:17
Hot Wire Anemometry
0 Views •
2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI Hot-wire anemometers have a very short time-response, which makes them ideal to measure rapidly fluctuating phenomena such as turbulent flows. The purpose of this experiment is to demonstrate the use of hot-wire anemometry.
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
0 Views •
Cited by 8 •
2012
Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
FAQs
Controlled resting tension establishes the mechanical starting condition for an isolated vessel segment before testing. Applying the same intended stretch across preparations helps researchers interpret subsequent increases or decreases in isometric force as responses to the experimental treatment rather than simply differences in initial setup. This is important when comparing vascular contractility or relaxation between biological samples.
Changes in recorded force after exposure to pharmacological agents provide separate evidence about how the vessel responds during contraction or relaxation. By examining these responses in the context of the experimental treatment, investigators can evaluate endothelial function and the responsiveness of vascular smooth muscle. The resulting measurements help connect cellular vascular behavior with broader changes in vessel function.
Isometric force recording quantifies how strongly a mounted vessel segment contracts or relaxes while its experimental arrangement is maintained. This produces a direct measurement of functional responsiveness rather than relying only on structural observations. In vascular biology, force changes can therefore be used to compare contractile behavior, relaxation, endothelial performance, and responses to pharmacological manipulation.
Researchers first isolate a small blood-vessel segment and mount it on fine wires in an organ bath. They then place the preparation in a physiological solution, apply a controlled resting tension, and expose it to selected pharmacological agents. Throughout the experiment, the system records changes in isometric force, which are analyzed as contractile or relaxing responses.
The core setup requires an isolated vessel segment, fine mounting wires, an organ bath, physiological solutions, and a means of recording isometric force. Controlled resting tension provides a defined starting condition, while pharmacological agents supply experimental stimuli. Together, these components maintain the vessel in a testable environment and allow its mechanical responses to be measured consistently.
This system is useful when researchers need functional information about small-vessel behavior rather than measurements limited to anatomy. Studies can use it to assess vasoconstriction, vasodilation, endothelial function, smooth muscle responsiveness, disease mechanisms, or drug effects. The recorded force responses help relate an experimental condition or treatment to changes in vascular performance.