Rem Linker Strategy
Rem Linker Strategy - Related Videos
10:34
Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
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Cited by 3 •
2013
A method of identifying unknown drivers of carcinogenesis using an unbiased approach is described. The method uses the Sleeping Beauty transposon as a random mutagen directed to specific tissues. Genomic mapping of transposon insertions that drive tumor formation identifies novel oncogenes and tumor suppressor...
01:09
Cytoskeletal Linker Proteins - Plakins
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2023
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
14:40
Expression Analysis of Mammalian Linker-histone Subtypes
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Cited by 22 •
2012
We describe a set of assays to analyze expression levels of H1 linker histones. mRNA of individual H1 genes are quantitatively measured by random primer based reverse transcription followed by real-time PCR, whereas protein quantification of H1 histones is achieved by HPLC analysis.
12:30
Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Cited by 12 •
2014
These studies report on reversible attachment of adenoviral gene vectors to coatless metal surfaces of stents and model mesh disks. Sustained release of transduction-competent viral particles contingent upon hydrolysis of cross-linkers used for vector immobilization results in a durable site-specific transgene expression in vascular cells and in stented arteries.
09:45
New Variations for Strategy Set-shifting in the Rat
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Cited by 3 •
2017
Set-shifting, a form of behavioral flexibility, requires an attentional shift from one stimulus dimension to another. We extended an established rodent set-shifting task1 by requiring attention to different stimuli according to context. The task was combined with specific lesions to identify neuron subtypes underlying a successful shift.
FAQs
Control comes from coordinating several linker properties rather than treating the connection as a passive attachment. Linker length and flexibility influence the distance and relative orientation of the connected units, while polarity affects solubility. Stability determines how persistently the connection remains intact, allowing chemists to tune the conjugate for its intended molecular function.
Length establishes the spatial separation between the connected units, whereas flexibility affects how freely those units can move relative to one another. Together, these features can influence whether the components adopt a useful orientation for molecular recognition or another designed function. Adjusting them gives researchers a way to optimize conjugate behavior without changing the linked components themselves.
Polarity is selected to help regulate the solubility of the resulting conjugate, which can affect how the molecule behaves in an experimental setting. Stability controls how long the connection persists. Considering both properties helps chemists balance handling and functional performance, especially when developing conjugates for probing, delivery, recognition, or therapeutic research.
Design begins by identifying the desired behavior of the conjugate, such as a particular distance, orientation, solubility, or persistence. Chemists then choose linker chemistry and structural features that address those requirements, including length, flexibility, polarity, and stability. The resulting construct can be optimized by examining activity, selectivity, and experimental control.
The strategy supports multifunctional molecules used in molecular recognition, delivery, probing, and therapeutic research. In each setting, the linker provides a means to adjust how connected components function together rather than simply joining them. This can help guide the development of chemical tools whose activity, selectivity, or experimental behavior is better suited to a specific research objective.
Purposeful control of linker architecture can improve activity, selectivity, and experimental control by regulating the relationship between connected units. These gains are important when a conjugate must perform more than one molecular role. In chemistry and chemical biology, systematic linker optimization therefore helps researchers refine multifunctional constructs and identify designs with more effective overall behavior.