Duplex Ultrasound
Duplex Ultrasound - Related Videos
10:41
Analysis of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage with High Frequency Transcranial Duplex Ultrasound
0 Views •
2021
The aim of this manuscript is to present a sonography-based method that allows in vivo imaging of blood flow in cerebral arteries in mice. We demonstrate its application to determine changes in blood flow velocities associated with vasospasm in murine models of subarachnoid hemorrhage (SAH).
04:01
Point of Care Transcranial Color-Coded Duplex Ultrasound of the Middle Cerebral Artery
0 Views •
Cited by 3 •
2024
Transcranial ultrasound is an essential tool for monitoring patients with various neurological conditions. Although it is commonly used in a protocolized fashion in consultative studies, the brain has been overlooked in many protocols utilizing point-of-care ultrasound (PoCUS). This study proposes a PoCUS image acquisition protocol.
11:42
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
0 Views •
2012
Crystal structure of protein–DNA complexes can provide insight into protein function, mechanism, as well as, the nature of the specific interaction. Here, we report how to optimize the length, sequence and ends of duplex DNA for co-crystallization with Escherichia coli SeqA, a negative regulator of replication initiation.
04:10
Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
0 Views •
2025
In this video, we demonstrate the procedure to determine the helicase activity of a target protein to unwind the biotin-labeled dsRNA substrate. The activity of the enzyme was identified by analyzing the electrophoretic mobility shift, followed by a chemiluminescence assay using chemiluminescent enzyme-conjugated streptavidin.
08:45
Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers
0 Views •
2025
In this video, we demonstrate the duplex digital PCR (ddPCR) technique — a modification of the traditional PCR technique that is useful in detecting two different genetic markers simultaneously. A single PCR reaction is partitioned into nanoliter-sized emulsified droplets that are independently amplified, and the detection of differently colored fluorescence amplification signals from the fraction of droplets is used to compute the initial concentration of the target sequences.
FAQs
The two outputs provide complementary clinical information. B-mode imaging shows real-time grayscale views of vascular structures, while Doppler analysis evaluates frequency shifts produced by moving blood cells. Interpreting them together lets clinicians relate a vessel’s anatomical appearance to blood-flow direction and velocity, helping connect structural abnormalities with their hemodynamic significance.
Frequency shifts generated by moving blood cells provide information about flow direction and velocity. In a vascular examination, these measurements add hemodynamic context to the grayscale image and help characterize abnormal circulation. Doppler analysis therefore does more than locate a vessel; it contributes information about how blood is moving through that vessel.
Linking the two data types helps clinicians interpret vascular disease in context. Grayscale imaging can show the vessel and its structural features, while Doppler measurements indicate associated blood-flow direction and velocity. Together, these findings support assessment of narrowing, blockage, aneurysms, thrombosis, and abnormal circulation, rather than relying on anatomy or flow alone.
Duplex Ultrasound is noninvasive and does not use ionizing radiation, distinguishing it from imaging approaches that depend on that form of energy. This characteristic supports its clinical role in evaluating vascular structures and circulation without ionizing radiation exposure. The technique can contribute to diagnosis, treatment planning, and monitoring when vascular information is needed.
It can evaluate arteries and veins for several clinically important abnormalities, including narrowing, blockage, thrombosis, aneurysms, and abnormal circulation. Because the examination combines structural visualization with flow assessment, its findings can address both the condition of the vessel and the movement of blood through it, making the method relevant across multiple vascular disease presentations.
Clinical examinations may focus on carotid, peripheral, or abdominal vessels, depending on the vascular area being investigated. In each setting, the paired information remains relevant: B-mode provides real-time structural views, while Doppler analysis indicates flow direction and velocity. This allows the technique to support evaluation across distinct vascular regions rather than one anatomical site.
Clinicians use the resulting anatomical and hemodynamic information to support diagnosis, guide treatment planning, and monitor vascular disease over time. The method is useful when the objective extends beyond detecting a structural abnormality to understanding its circulation-related significance. Its clinical role therefore spans initial evaluation and continuing assessment of affected arteries and veins.