Heparinized Syringe
Heparinized Syringe - Related Videos
02:21
Purification of Viral Integrase Using Heparin Affinity Chromatography
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2026
Source: Lopez Jr., M. et al. Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase. J. Vis. Exp. (2017)This video demonstrates the procedure for purifying viral integrase from bacterial nuclease contamination using heparin Sepharose column chromatography followed by SDS-PAGE analysis to confirm protein purity.
12:27
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
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Cited by 2 •
2016
Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.
10:41
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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Cited by 2 •
2017
A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.
01:30
Anticoagulant Drugs: Low-Molecular-Weight Heparins
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2024
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
02:28
Extraction of Apoplastic Bacteria Using a Syringe-Based Pressure Method
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2025
Source: Rufián, J. S., et al, Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a syringe-based pressure method for extracting apoplastic bacteria, which proliferate and form microcolonies within the apoplast, the intercellular space between plant cells. The method yields debris-free bacterial samples suitable for downstream single-cell analysis.
FAQs
Heparin supports anticoagulation by enhancing antithrombin activity. Antithrombin then inhibits key clotting factors, including thrombin and factor Xa, reducing the likelihood that collected blood will form a clot. This mechanism preserves a fluid specimen rather than allowing clot formation to interfere with the sample, which is particularly important when the specimen must support arterial blood gas measurements.
A fluid sample is necessary because arterial blood gas analysis measures oxygen, carbon dioxide, and acid–base status. If clot formation changes the specimen, the sample may no longer support reliable measurement of these variables. Thus, anticoagulation is not merely a storage feature; it protects the sample condition required to assess respiratory and metabolic function accurately.
Clots can interfere with maintaining a fluid sample, creating a source of error during arterial blood gas analysis. Because the analysis is used to measure oxygen, carbon dioxide, and acid–base status, preserving sample integrity directly supports reliable assessment of respiratory and metabolic function. Avoiding these errors depends on proper syringe selection, mixing, and prompt analysis.
A practical sequence is to select the appropriate heparinized syringe, collect blood under controlled conditions, mix the sample properly, and arrange prompt analysis. Each step supports sample integrity: selection provides the intended collection device, mixing maintains the prepared specimen, and timely analysis helps limit clot-related problems. Together, these measures improve the reliability of arterial blood gas assessment.
Prompt analysis helps reduce clot-related errors after blood has been collected in the syringe. This is especially relevant when the specimen is intended for arterial blood gas analysis, because the sample must remain fluid for measurement of oxygen, carbon dioxide, and acid–base status. Timely handling therefore supports reliable evaluation of respiratory and metabolic function.
Heparinized syringes are particularly relevant when clinicians perform arterial blood gas analysis. This application requires a preserved, fluid blood specimen so oxygen, carbon dioxide, and acid–base status can be measured accurately. The resulting information supports assessment of respiratory and metabolic function, making correct syringe selection, mixing, and prompt analysis important parts of the clinical workflow.