Respiratory Diagnostics
Respiratory Diagnostics - Related Videos
09:57
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
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Cited by 11 •
2016
Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.
14:56
Remote Laboratory Management: Respiratory Virus Diagnostics
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Cited by 9 •
2019
A rapidly-deployable, off-grid laboratory has been designed and built for remote, resource-constrained global settings. The features and critical aspects of the logistically-enhanced, expandable, multifunctional laboratory modules are explored. A checklist for a basic laboratory workflow and a protocol for a respiratory viral diagnostic test are developed and presented.
06:22
Machine Learning-Based Cough Tone Classification: Diagnostic Exploration of Chronic Obstructive Pulmonary Disease and Respiratory Tract Infections
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2025
This study effectively accomplished the automated classification of two distinct categories by acquiring cough sound data from patients diagnosed with chronic obstructive pulmonary disease (COPD) and respiratory tract infections (RTI), utilizing an integration of speech signal processing techniques and machine learning algorithms.
07:12
Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
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Cited by 7 •
2014
Diagnostic ultrasound imaging has proven to be effective in diagnosing various respiratory diseases in human and animal subjects. We demonstrate a comprehensive ultrasound protocol utilized by Dr. Zuo's lab to analyze diaphragm kinetics specifically in mouse models. This is also a non-invasive research technique which can provide quantitative information on mouse respiratory muscle function.
11:00
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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Cited by 11 •
2016
High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.
FAQs
Each measurement addresses a different aspect of lung performance. Airflow and lung-volume results describe how air moves through and occupies the lungs, while gas-exchange measurements and blood oxygen saturation indicate how effectively oxygen-related respiratory function is being maintained. Imaging adds structural information. Considering these results together helps clinicians classify abnormalities rather than relying on a single signal.
Spirometry provides objective information about airflow and lung volume, allowing clinicians to evaluate functional changes in the respiratory system. Its results can support identification and classification of breathing problems and provide a reference for follow-up assessments. Repeated measurements may also show whether lung function changes over time or responds to treatment, which strengthens clinical monitoring.
Objective measurements show what is happening in airflow, lung volume, gas exchange, oxygen saturation, or lung structure, but they do not replace clinical context. Patient history helps clinicians interpret those findings in relation to breathing problems and possible causes. This combined assessment supports more informed detection, classification, treatment decisions, and evaluation of disease progression.
These methods provide complementary evidence. Imaging examines respiratory structure, spirometry evaluates airflow and lung volume, and pulse oximetry measures blood oxygen saturation. A clinician can compare structural findings with functional and oxygen-related measurements to build a more complete assessment. This combination is useful when evaluating respiratory conditions or determining whether further monitoring and treatment decisions are needed.
The process begins with a patient history and selection of tests suited to the breathing problem being evaluated. Clinicians may obtain functional measurements, assess oxygen saturation, or use imaging to examine lung structure. They then interpret the findings together, classify the respiratory issue, and establish measurements that can be compared during later assessments or after therapy.
Monitoring is valuable when clinicians need to track disease progression or determine whether a patient is responding to therapy. Serial measurements can reveal changes in airflow, lung volume, gas exchange, oxygen saturation, or imaging findings. In clinical care, this information supports timely adjustment of treatment decisions and helps evaluate conditions such as asthma, chronic obstructive pulmonary disease, infections, and respiratory failure.