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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

DOI:
10.3791/54781-v

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11:00 min

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November 28, 2016

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, , , , , , , , ,
1Population Medicine and Diagnostic Sciences,Cornell University Animal Health Diagnostic Center, 2Thermo Fisher Scientific Inc.

Chapters

  • 00:05Title
  • 01:00Reverse-transcription/Pre-amplification (Preamp)
  • 03:47Liquid Handler Transfer
  • 07:58Result Analysis
  • 09:15Results: Representative Results from an RNA Assay, Influenza Virus A, and a DNA Assay, Equine Herpesvirus Type 1
  • 10:25Conclusion

Summary

Automatic Translation

Automatic Translation

High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.

Tags

Keyword Extraction: High-throughput DetectionRespiratory PathogensAnimal SpecimensNanoscale PCRReal-time PCRDNARNAPathogensOneHealth SurveillanceInfectious DiseasesClinical SpecimensRespiratory PathogensHorsesDogsLysis BufferReverse TranscriptionPre-amplificationPreamp96-well PlateThermocyclerCDNA Production

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