Skin Application
Skin Application - Related Videos
09:42
Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research
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2017
This protocol is a cost-effective alternative for expressing naked plasmid DNA in mouse skin. The overall goal of the protocol is to deliver immune-related genes into skin tissue to delineate the functional role of a specific gene in cutaneous inflammation.
13:58
Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
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Cited by 26 •
2014
Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.
07:15
Standardized Method to Detect Tunneling Nanotubes in Human Skin Cells for Tissue Engineering Applications
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2026
Here, a standardized protocol to visualize tunneling nanotubes between human epidermal keratinocytes and dermal fibroblasts using membrane and F-actin labeling with z-stack confocal imaging is shown, with optional α-tubulin co-staining, enabling reproducible detection and cytoskeletal characterization for tissue-engineering applications.
06:42
Murine Skin Transplantation
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Cited by 36 •
2008
Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c-->C57BL/6 skin transplant.
05:44
Skin Biopsy for Diagnosing Discoid Lupus Erythematosus
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Cited by 2 •
2025
Skin lesions are common and important features in many rheumatic diseases, and biopsy with pathological analysis plays a critical role in diagnosis. This article provides a detailed overview of the standardized skin biopsy procedure and, using discoid lupus erythematosus as an example, highlights the essential role of dermatopathology in diagnosing rheumatic diseases.
FAQs
The stratum corneum acts as the skin’s main outer barrier, so it strongly influences whether an active compound remains near the surface or diffuses into deeper tissue. Formulations must interact with this barrier in a way that supports the intended effect. Differences in barrier interaction help explain variation in local activity, tissue retention, and absorption between products.
Formulation affects how an active compound interacts with the stratum corneum and whether it remains within the tissue or moves through it. Contact time also influences the opportunity for these processes to occur. Consequently, the same active compound may produce different therapeutic outcomes when formulation, exposure duration, or other application conditions change.
Local treatment aims to produce its primary effect at or near the application site, whereas transdermal delivery relies on movement through the skin to support delivery into the body. The distinction depends on where the active compound acts and how far it diffuses. This difference guides clinical selection for localized conditions versus systemic delivery goals.
Clinicians should consider the intended effect, the formulation, the dose, contact time, and the condition of the skin. These factors influence how much of an active compound remains in tissue or is absorbed through the barrier. Matching product characteristics and application conditions to the clinical goal supports appropriate treatment selection and safer use.
In wound care and localized conditions, clinicians can select products intended to act at the affected skin or tissue rather than relying on delivery throughout the body. The desired local effect depends on formulation, dose, contact time, and skin condition. Evaluating these variables helps align application with the treatment goal and expected therapeutic outcome.
Assessment should relate the observed therapeutic result to the intended delivery pattern, whether local tissue activity or movement into the body. Formulation, dose, contact time, and skin condition can each alter absorption and effectiveness, so an unexpected outcome may reflect application conditions as well as the active compound. This context supports safer clinical decisions.