Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

Hao Chang; Yanshu Wang; Hao Wu; Jeremy Nathans

doi:10.3791/51749

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Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

21,245 Views

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13:58 min

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June 25, 2014

DOI:

10.3791/51749-v

DOI:

10.3791/51749-v

•

13:58 min

June 25, 2014

•

22 Views

Hao Chang¹, Yanshu Wang¹, Hao Wu¹, Jeremy Nathans^1,2,3

¹Department of Molecular Biology and Genetics,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, ²Department of Neuroscience,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, ³Department of Ophthalmology,Howard Hughes Medical Institute, Johns Hopkins University School of Medicine

Summary

Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.