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Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology

Article DOI: 10.3791/51749 • 13:58 min • June 25th, 2014
June 25th, 2014 •
1, 1, 1, 1,2,3
1Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 2Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 3Department of Ophthalmology, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine

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Summary
Automatic Translation

Mammalian skin contains a diverse array of structures - such as hair follicles and nerve endings - that exhibit distinctive patterns of spatial organization. Analyzing skin as a flat mount takes advantage of the 2-dimensional geometry of this tissue to produce full-thickness high-resolution images of skin structures.

Tags

Flat Mount Imaging Mouse Skin Hair Follicle Patterning Sensory Axon Morphology Intra-dermal Structures Skin Visualization 2-dimensional Geometry Dissection Fixation Permeabilization Staining Clarification Flat Mount Protocol Optical Sectioning Reconstruction Position And Orientation Analysis
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