Human Corneal Fibroblasts
Human Corneal Fibroblasts - Related Videos
03:33
Generating a 3D Co-Culture Model of Human Corneal Fibroblasts and Neurons
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2025
This video demonstrates a technique to generate a three-dimensional co-culture model system of primary human corneal fibroblasts and differentiated neuronal cells. This co-culture system can be used to study the stromal-nerve interactions.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
03:32
Generating Neurons by Reprogramming Human Dermal Fibroblast Cells
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2025
The video demonstrates the generation of neurons from adult human dermal fibroblasts (aHDFs). The aHDFs are reprogrammed using a lentiviral vector carrying genes for neuronal transcription factors and short hairpin RNAs (shRNAs). The shRNAs inhibit the expression of a neuronal transcriptional repressor, and the neuronal transcription factors activate genes to differentiate aHDFs into neurons. These reprogrammed aHDFs are cultured in an early and a late neuronal conversion medium to generate...
06:02
Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens
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Cited by 17 •
2016
Here, a protocol to isolate and establish primary fibroblast/myofibroblast (MF) cultures from frozen gastric, small intestinal, and colonic tissue-yielding cells with a MF phenotype-is presented. These cells express CD90, α-SMA and vimentin. MFs can be used for a variety of functional assays including enzymatic activity and cytokine production.
10:04
Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
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Cited by 2 •
2017
Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.
FAQs
Transforming growth factor beta can shift relatively quiescent keratocytes toward an activated fibroblast state. Under some conditions, activation progresses toward contractile myofibroblasts, which produce and reorganize extracellular matrix. This signaling response is important because it links biochemical cues to changes in cell behavior, matrix remodeling, and the balance between corneal repair and scar formation.
The extracellular matrix provides the structural basis for corneal stromal strength and transparency. Human corneal fibroblasts contribute by producing and reorganizing collagen and other matrix components, so their activity affects both tissue architecture and optical properties. Developmental studies can therefore examine cell-matrix interactions to understand how stromal organization is established, maintained, or altered after injury.
These cell states represent different functional responses within the corneal stroma. Keratocytes are relatively quiescent, activated fibroblasts participate in matrix production and remodeling, and myofibroblasts can display contractile behavior while reorganizing matrix. Comparing these states helps researchers relate biochemical or injury-associated signals to distinct outcomes, including normal repair, persistent remodeling, and scar formation.
These models help investigators examine how the corneal stroma forms and how stromal cells interact with their surrounding matrix. They can connect cellular behavior with tissue-level features such as strength and transparency, while also revealing how signaling and matrix remodeling influence later responses to injury. This makes the cells relevant to both developmental processes and regenerative research.
Cultured cells provide an experimental system for studying fibroblast activation, extracellular-matrix production, and cell-matrix interactions under defined research conditions. Investigators can use these models to examine responses to biochemical signals such as transforming growth factor beta and to explore mechanisms associated with repair or scarring. The resulting observations support disease studies and evaluation of corneal repair strategies.
They are especially useful when the research question concerns how stromal cells respond after injury. Their transitions between relatively quiescent, activated, and contractile states allow investigators to relate cellular activation to collagen production, matrix reorganization, and scar formation. This framework helps distinguish processes that may support repair from responses that can disrupt corneal transparency.
In tissue-engineering models, these cells help researchers investigate how stromal cells contribute to matrix formation and organization within a repair-oriented construct or experimental system. Their behavior can provide information about cell-matrix interactions, stromal development, and remodeling. Such models support research into corneal repair and regenerative therapies without relying solely on observations from damaged tissue.