Monodisperse Microdroplet Formation
Monodisperse Microdroplet Formation - Related Videos
08:20
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
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Cited by 9 •
2016
Here, we demonstrate a simple production method for size-controllable, monodisperse, water-in-oil (W/O) microdroplets using a capillary-based centrifugal microfluidic device. This method requires only a small sample volume and enables high-yield production. We expect this method will be useful for rapid biochemical and cellular analyses.
03:20
Real-Time Monitoring of Bacterial Growth Using a Microfluidic Microdroplet Culture System
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2025
Source: Jian, X. et. al., Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC). J. Vis. Exp. (2022)This video demonstrates the use of a microfluidic droplet-based platform for real-time monitoring of bacterial growth. By generating uniform droplets containing bacterial suspensions and guiding them through a detection pathway, the system enables continuous optical measurement of cell density to construct a growth curve.
03:42
Adaptive Evolution of Bacteria Using a Microbial Microdroplet Culture System
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2025
Source: Jian, X., et al., Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC). J. Vis. Exp. (2022).This video demonstrates the use of a microbial microdroplet culture system to drive adaptive evolution of bacteria toward methanol tolerance. By mixing engineered bacterial cultures with a high-methanol stress medium and segmenting them into microdroplets, the system selects for mutants that exhibit improved growth under methanol stress. Through...
02:16
Solution Formation
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2020
There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride. This selective solubility...
02:37
Standard Enthalpy of Formation
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2020
Enthalpy changes are typically tabulated for reactions in which both the reactants and products are at the same conditions. A standard state is a commonly accepted set of conditions used as a reference point for the determination of properties under other different conditions. For chemists, the IUPAC standard state refers to materials under a pressure of 1 bar and solutions at 1 M and does not specify a temperature. Many thermochemical tables list values with a standard state of 1 atm. Because...
FAQs
Breakup occurs when the immiscible continuous phase exerts shear on the dispersed phase and pinches it at the channel junction. This interfacial deformation separates one portion of fluid from the incoming stream. Because the event is governed within a defined microchannel location, engineers can connect breakup behavior to operating conditions and design a repeatable droplet-production process.
These variables regulate how readily the dispersed phase deforms and detaches. Flow rates determine the relative movement of the two phases, interfacial tension governs resistance at their boundary, and channel geometry shapes the location and manner of pinching. Adjusting them changes droplet size and supports engineering control over the resulting microdroplet population.
Uniform droplet volumes make fluid compartments more consistent from one droplet to the next. That consistency improves reproducibility when droplets serve as reaction spaces, synthesis environments, or assay compartments. It also strengthens process control because observed differences are less likely to arise from uncontrolled variation in compartment size, supporting more reliable engineering and analytical outcomes.
A dispersed phase and an immiscible continuous phase are introduced into a microfluidic device containing a channel junction. The continuous phase then shears or pinches the dispersed phase, producing separated droplets. Engineers regulate the relevant flow rates and use the channel geometry and interfacial conditions to obtain the intended droplet size and consistency.
The process requires a microfluidic device, a channel junction, and two immiscible fluid phases: a continuous phase and a dispersed phase. The principal operating controls are the phase flow rates, interfacial tension, and channel geometry. Together, these components and conditions determine how the dispersed phase breaks up and how uniform the resulting droplets become.
Engineers apply controlled microdroplet production to compartmentalized chemical reactions, particle synthesis, emulsion production, and high-throughput biological assays. In these settings, consistent droplets provide controlled fluid compartments and improve reproducibility. The same platform also supports functional-material design, mass-transfer optimization, and automated analytical systems, making it useful across both process engineering and laboratory-scale technologies.