JoVE Encyclopedia of Experiments
Microbiology
0 views • 3:42 min • October 30th, 2025
Inject a bacterial culture engineered for methanol tolerance into one reagent bottle and a stress medium with high methanol concentration into another.
Add oil to both and place them in a microbial microdroplet culture system to reach the incubation temperature.
Using a pump, push the liquids through a microfluidic chip.
The bacterial culture mixes with the stress medium. The oil separates the mixture into microdroplets, which move back and forth for cultivation.
Initially, high methanol stress slows bacterial growth.
Over time, some cells acquire mutations that improve methanol utilization and proliferate.
The system measures bacterial density and selects droplets showing higher growth.
These are remixed with the stress medium to enrich for greater methanol utilization.
After several cycles, extract droplets with high bacterial density, spread them on agar, and incubate.
Isolate and culture the formed colonies in a liquid medium to obtain methanol-adapted strains.
Inject required volumes of the initial bacteria solution, fresh medium, and MMC oil into the separate sterilized reagent bottles for the initial bacteria solution in the fresh medium, as explained earlier.
To choose the function of
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