Sample Extraction Method

Sample extraction methods are procedures that isolate target substances from environmental matrices such as water, soil, sediment, or air so they can be measured accurately. They work by transferring analytes from a complex matrix into a suitable extract, using differences in properties such as solubility, polarity, volatility, or chemical affinity; filtration, phase separation, or concentration may then improve measurement. In environmental science, extraction supports the detection of pollutants, nutrients, metals, and other chemical indicators during monitoring and risk assessment. Reliable extraction improves analytical sensitivity and comparability, helping researchers evaluate contamination, track environmental change, and guide remediation decisions.

Sample Extraction Method - Related Videos

Research

JoVE EoE - Lung Cancer

miRNA Extraction: A Method to Extract miRNA from Plasma Sample

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2023

MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.

Research

JoVE Journal - Environment
Free Sample

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

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Cited by 20 •

2017

The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.

Circulating miRNA Extraction: A Method to Isolate miRNA from Plasma Samples Via Organic Extraction and Small RNA Enrichment

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2023

This video describes the extraction of circulating microRNA from plasma samples of colorectal cancer patients using organic extraction followed by glass-fiber column enrichment. miRNAs are stable in biological fluids such as serum and plasma, which renders them ideal circulating biomarkers for cancer diagnosis, prognosis, and treatment decision-making and monitoring. This method will help identify new circulating biomarkers capable of predicting clinical outcomes in patients.

Research

JoVE Journal - Biochemistry
Free Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

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Cited by 67 •

2017

A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

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Cited by 2 •

2016

A protocol for the extraction and pre-concentration of estradiol from water samples by using an automated and miniaturized system is presented.

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