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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

DOI:

10.3791/55802-v

•

11:17 min

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June 01, 2017

•

Mohamed Salem², Michal Bernach, Krzysztof Bajdzienko, Patrick Giavalisco

¹Max Planck Institute of Molecular Plant Physiology, Golm, Germany, ²Department of Pharmacognosy, Faculty of Pharmacy,Cairo University

Chapters

00:05Title
01:05Grinding and Tissue Disruption
02:30Aliquoting Tissue
03:18Extraction and Fractionation by Phase Separation
04:56Aliquoting Polar and Hydrophobic Fractions
06:24Concentrations and Storage of Fraction and Analysis of Lipids Using UPLC-MS
07:48Analysis of Polar and Semi-polar Metabolites Using UPLC-MS
08:51Results: Representative Chromatograms of Lipid, Semi-polar Metabolites and Proteins from Arabidopsis thaliana Leaf Extracts
10:40Conclusion

Summary

Automatic Translation

A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.

Tags

Keywords: Fractionated Extraction Metabolites Lipids Proteins Single Sample MTBE Extraction Multiomics Analysis Arabidopsis Sample Preparation

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