Mouse Skin Epithelium
Mouse Skin Epithelium - Related Videos
03:38
Isolation of Circumvallate Papillae (CVP) Epithelium from Mouse Model: An Enzymatic Procedure to Separate CVP Epithelium from Mouse Tongue
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2025
In this video, we describe the isolation of circumvallate papillae (CVP) epithelium from a murine tongue.
03:42
Rapid Dissection and Dissociation of the Mouse Olfactory Epithelium for Single-Nucleus Suspensions
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Cited by 1 •
2025
This article provides a streamlined protocol for the dissection and dissociation of murine olfactory epithelium for the purpose of single-nucleus RNA sequencing.
03:10
Direct-Coupled Electroretinogram Recording of the Retinal Pigment Epithelium in a Mouse
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2025
This video demonstrates the recording of a direct-coupled electroretinogram (DC-ERG) on a dark-adapted, anesthetized mouse retinal pigment epithelium (RPE). After applying dilating drops, the animal is placed in a recording setup, and the capillary electrode filled with a salt buffer is placed on the cornea. After establishing the baseline, DC-ERG is recorded.
16:47
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
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Cited by 45 •
2011
A formal demonstration of the dissection of a mouse eye, resulting in a whole mount of the retinal pigment epithelium.
06:56
Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium
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Cited by 4 •
2015
Mitochondrial dysfunction is a hallmark of cellular senescence. This paper uses non-invasive near-infrared (NIr) treatment to improve mitochondrial function in the aging mouse vestibular sensory epithelium.
FAQs
Basal keratinocytes contribute to tissue maintenance through a coordinated sequence of division, differentiation, and migration. Newly produced cells move toward the surface while acquiring features associated with the mature epithelial barrier. Studying this progression allows researchers to assess how genes regulate cell renewal and organization, both during normal turnover and when epithelial repair is required.
Resident stem cells provide a replenishing cell source when ordinary tissue turnover is insufficient. Following injury, their contribution can be examined alongside keratinocyte division, differentiation, and migration to determine how the epithelium restores coverage. Genetic analysis of these responses helps connect particular genes with the capacity to maintain tissue integrity and support repair.
Keratin production and specialized cell junctions contribute complementary structural functions as epithelial cells move toward the surface. Keratin helps establish the material properties of mature cells, while junctions support connections between neighboring cells. Examining both features helps reveal how genetic changes affect epithelial structure and the formation of a functional protective barrier.
A genetic manipulation can be evaluated by examining resulting changes in epithelial structure, signaling, maintenance, repair, or disease-related phenotypes. Comparing these outcomes with the expected tissue behavior helps researchers associate a gene with a specific biological role. This strategy turns visible or measurable epithelial changes into evidence about gene function in tissue biology.
These models support studies of gene regulation, epithelial development, wound healing, carcinogenesis, and inherited skin disorders. The same tissue system can therefore address both normal biology and disease mechanisms. Researchers may focus on how altered gene activity changes tissue maintenance or repair, or on how genetic differences produce structural and pathological phenotypes.
Researchers can use genetic manipulation to associate particular genes with epithelial abnormalities and disease phenotypes relevant to inherited skin disorders. Examination of structure, signaling, tissue maintenance, and repair provides several levels of evidence rather than relying on a single visible trait. This makes the model useful for connecting genotype with epithelial consequences.