Hippocampal Cannulation
Hippocampal Cannulation - Related Videos
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
04:28
Cannulation of the Carotid Artery in a Rat Model
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2025
In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
08:20
Organotypic Hippocampal Slice Cultures
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Cited by 52 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
FAQs
The implanted guide provides a stable access route, so researchers can return to the hippocampal region across multiple experimental time points without creating a new surgical pathway each time. This reduces additional tissue disruption and makes it possible to compare localized interventions or measurements over time, strengthening studies of evolving neural, immune, or infection-related responses.
Localized administration helps distinguish effects produced within the hippocampal region from broader consequences of exposing the entire brain or body to a compound. This focus is valuable when investigators examine pathogens, immune mediators, or therapeutic agents and need to relate a targeted manipulation to changes in hippocampal tissue, host responses, or behavior.
The access route can support both administration and sample collection, allowing investigators to manipulate the hippocampus and assess local responses under controlled conditions. When combined with analyses of neuroinflammation and host responses, these measurements can help connect a regional brain event with immune activity and clarify how central nervous system and immune processes interact.
A study generally includes surgical placement of the guide cannula, recovery or preparation under the investigator's controlled conditions, and repeated administration or collection through the implanted guide. Researchers then compare resulting tissue, immune, infection-related, or behavioral measurements across experimental conditions and time points. The overview supports these stages but does not specify anesthesia, coordinates, or hardware details.
It is useful when the research question requires direct, region-specific examination of how a pathogen, immune mediator, or therapeutic agent affects the hippocampus. Investigators can follow localized changes over time and relate them to neuroinflammation, host responses, or behavior. This creates a framework for studying communication between immune processes and the central nervous system.
Behavioral findings can be evaluated alongside targeted hippocampal manipulation and local measurements rather than treated as isolated whole-animal effects. If a compound or infection-related process is delivered to, or sampled from, the hippocampal region, investigators can examine whether associated tissue and immune responses occur together with behavioral changes. This supports more specific interpretation of brain-immune interactions.