Mdck A549 Cells
Mdck A549 Cells - Related Videos
07:25
Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
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Cited by 5 •
2015
We present a protocol describing a semi-quantitative method for measuring both, the attachment of influenza A virus to A549 cells, as well as the internalization of virus particles into the target cells by flow cytometry.
10:14
Generation of 3D Collagen-Embedded A549/MRC-5 Alveolar Organoids using a Mechanical Cell-Stretching Bioreactor
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2026
This protocol outlines the generation of organoids from A549 alveolar epithelial cells and MRC-5 lung fibroblasts on an extracellular matrix hydrogel scaffold, followed by embedding in 3D collagen gels and exposure to mechanical strain using a cell-stretching bioreactor to investigate alveolar mechanobiology.
12:09
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
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Cited by 28 •
2017
Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.
03:34
Assessing Caspase-Mediated Cleavage of Viral and Host Proteins in Virus-Infected Cells
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2025
The video demonstrates a method to assess caspase-mediated protein cleavage in virus-infected cells. Post-viral infection, caspases get activated and degrade various host cellular proteins and viral nucleoproteins while specific caspase inhibitors prevent this degradation, as confirmed by western blotting.
09:38
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
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Cited by 5 •
2011
This article details the procedures involved in overexpression and analysis of small GTPases in polarized epithelial cells using microinjection technique.
FAQs
MDCK and A549 provide complementary context because their canine kidney and human lung origins allow host-pathogen behavior to be examined in distinct epithelial settings. Comparing results across the two systems can reveal whether differences in pathogen entry, replication, antigen expression, or cytopathic effects are associated with the cellular model. This comparison supports interpretation of tissue-related infection patterns.
An adherent monolayer allows infection to be examined across an organized epithelial layer rather than as isolated cells. In MDCK and A549 cultures, this arrangement supports investigation of epithelial barrier behavior alongside pathogen entry, replication, antigen expression, and cytopathic effects. It therefore provides a framework for linking cellular organization with measurable infection outcomes.
Entry, replication, antigen expression, and cytopathic effects provide distinct readouts of infection-related change. Examining these outcomes together helps researchers determine whether a model shows initial pathogen interaction, evidence of ongoing replication, detectable pathogen-associated antigen, or visible cellular injury. That layered assessment improves interpretation of infection dynamics without relying on a single measurement.
A typical experiment begins by growing MDCK or A549 cells as an adherent monolayer. Researchers then expose the culture to a virus or another infectious agent and evaluate selected outcomes, such as pathogen entry, replication, antigen expression, or cytopathic effects. The workflow can be adapted to compare cell types or examine infection dynamics.
They can serve as infection models in which treatment-related changes are assessed against measurable infection outcomes. Investigators may examine whether pathogen replication, antigen expression, or cytopathic effects differ under the screening conditions. Using both cell lines can add complementary epithelial contexts, helping determine whether an observed effect is consistent across models.
They connect epithelial biology with host-pathogen interactions by enabling study of epithelial barriers and innate immune responses during infection. A549 cells provide a human lung context, while MDCK cells add a distinct canine epithelial context. Together, they support investigation of respiratory and systemic infection mechanisms, pathogen characterization, and cellular factors that influence infection outcomes.