Intestinal Immunity
Intestinal Immunity - Related Videos
05:34
Oral Delivery of Transformed Yeast Cells into the Mouse Intestinal Immune System
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2025
This video showcases the oral administration of transformed yeast cells into a mouse model. The transformed yeast cells are orally administered using a gavage technique, reaching the mouse's stomach. Later, the cells migrate to the small intestine, where microfold cells engulf them and facilitate their transfer to Peyer's patches and gain access to the intestinal immune system.
07:39
Basic Three-Dimensional (3D) Intestinal Model System with an Immune Component
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Cited by 5 •
2023
Here we describe constructing a basic three-dimensional (3D) intestinal cell line model system and a paraffin embedding protocol for light microscopic evaluation of fixed intestinal equivalents. Staining of selected proteins permits the analysis of multiple visual parameters from a single experiment for potential use in preclinical drug screening studies.
11:28
Isolating Lymphocytes from the Mouse Small Intestinal Immune System
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Cited by 59 •
2018
Here we describe a detailed protocol for the isolation of lymphocytes from the inductive sites including the gut-associated lymphoid tissue Peyer's patches and the draining mesenteric lymph nodes, and the effector sites including the lamina propria and the intestinal epithelium of the small intestinal immune system.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
08:48
Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses
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Cited by 3 •
2024
Our detailed protocol outlines the creation and use of the advanced intestine-on-chip model, which simulates human intestinal mucosa with 3D structures and various cell types, enabling in-depth analysis of immune responses and cellular functions in response to microbial colonization.
FAQs
Mucus creates a protective interface over the intestinal epithelium, while antimicrobial peptides provide chemical defenses against invading organisms. Together, these components help reduce direct contact between microbes and epithelial surfaces and contribute to barrier integrity. Their activity is important because protection must limit pathogens without eliminating the beneficial microbial communities that coexist in the gastrointestinal tract.
Innate immune sensors detect microbial signals and help initiate early protective responses, whereas secretory immunoglobulin A provides a more targeted adaptive defense. Immunoglobulin A can limit microbial attachment and help neutralize invading organisms. This division of labor allows intestinal immunity to respond rapidly while also controlling interactions between microbes and the epithelial environment.
The gut microbiota continuously interacts with intestinal tissues and immune cells, influencing whether responses favor tolerance or inflammation. This relationship helps explain how the intestine accommodates beneficial microbes while remaining capable of responding to harmful organisms. Disruption or imbalance in these interactions is therefore relevant to research on inflammatory conditions and other diseases involving intestinal immune regulation.
In enteric infections, investigators can examine how epithelial defenses, innate sensing, antimicrobial peptides, and immunoglobulin A limit invading organisms. In inflammatory bowel disease, the focus shifts toward how immune cells, intestinal tissues, and microbiota interactions contribute to excessive inflammation. Comparing these settings helps connect protective mechanisms with pathological responses and identifies processes relevant to disease research.
Food allergy research examines how intestinal immune responses distinguish harmless dietary material from threats, while microbiome-based therapy research considers how beneficial microbial communities may influence immune regulation. Both areas depend on understanding interactions among intestinal tissues, immune cells, and the microbiota. This context makes intestinal immunity central to investigating tolerance, inflammation, and potential therapeutic strategies.
Mucosal vaccine research relies on understanding how immune defenses operate at intestinal surfaces, including antigen interactions with epithelial tissues and adaptive responses such as secretory immunoglobulin A. These responses can help limit microbial attachment and neutralize invading organisms. Studying intestinal immunity therefore provides scientific context for designing and evaluating vaccine approaches intended to protect through gastrointestinal or other mucosal sites.