Journal
/
/
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

30,468 Views

10:58 min

April 20, 2015

DOI:

10:58 min
April 20, 2015

13 Views
, , , ,

Summary

Automatically generated

In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.

Related Videos

Read Article