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10:58 min
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April 20, 2015
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Summary
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In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
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Cite this Article
Van Lidth de Jeude, J. F., Vermeulen, J. L. M., Montenegro-Miranda, P. S., Van den Brink, G. R., Heijmans, J. A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids. J. Vis. Exp. (98), e52531, doi:10.3791/52531 (2015).
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