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A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

DOI:
10.3791/52531-v

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10:58 min

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April 20, 2015

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, , , ,
1Tytgat Institute for Liver and Intestinal Research, Department of Gasteroenterology and Hepatology,Academical Medical Center

Chapters

  • 00:05Title
  • 01:39Production of Lentiviral Praticles
  • 04:30Lentiviral Transduction of Organoids
  • 07:04Processing Organoids for Paraffin Embedding and Immunohistochemistry
  • 09:21Analysis of Lentivirus-transduced Organoids
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.

Tags

Keywords: Intestinal OrganoidsLentiviral TransductionGene OverexpressionGene KnockdownQuantitative RT-PCRRNA-microarrayImmunohistochemistryEpithelial Cell Culture

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