Murine Kidney Isolation
Murine Kidney Isolation - Related Videos
03:35
Glomeruli Isolation: A Technique to Obtain Intact Glomeruli from Murine Kidney
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2023
This video describes the method to isolate intact glomeruli from mouse kidneys. The encapsulated and decapsulated glomeruli thus obtained can further be used for culturing parietal epithelial cell outgrowths from the murine kidney.
17:42
A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
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Cited by 134 •
2011
Transplantation of isolated islets has been proposed to be a potential treatment for type 1 diabetes. Here we describe a method to isolate islets from mouse pancreata and transplant them to the subcapsular space of the kidney.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
03:14
Vibratome Sectioning of Whole Murine Kidney: A Technique to Generate Thin Sections of Murine Kidney
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2023
In this video, we demonstrate the vibratome sectioning of a whole mouse kidney to obtain thin slices. These sliced tissue sections can be stored at low temperatures until further downstream analysis.
03:00
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models
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2023
In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
FAQs
Maintaining tissue integrity keeps the recovered kidney suitable for multiple downstream analyses. Intact samples can retain structural features needed for histology and immunostaining, while preserved cellular material supports flow cytometry, culture, and molecular assays. Careful handling therefore allows researchers to compare tissue architecture, cellular composition, and immune or pathogen-associated changes from the same experimental system.
Mechanical disruption breaks the isolated kidney into tissue fragments, whereas enzymatic digestion is used to generate a single-cell suspension. These preparations support different analytical needs: fragments can preserve tissue-level organization, while individual cells are more suitable for cellular characterization such as flow cytometry. Selecting the processing approach depends on whether structural or cell-based information is the main outcome.
Kidney samples can reveal renal inflammation, leukocyte infiltration, tissue injury, and broader host responses during infection or experimental disease. Histology and immunostaining provide tissue-level evidence, while cell suspensions support analysis of immune-cell populations. Together, these readouts connect visible renal damage with changes in the local immune response rather than treating infection effects as systemic findings alone.
The workflow begins with abdominal dissection, followed by careful removal of tissue surrounding the kidneys. The kidneys are then collected under conditions intended to preserve tissue integrity. After isolation, samples may remain as tissue or undergo mechanical disruption or enzymatic digestion, depending on whether the planned analysis requires structural specimens or single-cell suspensions.
The intended assay should determine how the kidney is processed after collection. Histology and immunostaining require tissue that retains relevant structure, whereas flow cytometry generally benefits from a single-cell suspension produced through enzymatic digestion. Mechanical disruption offers tissue fragments for analysis when complete cellular dissociation is not required, aligning sample preparation with the desired biological resolution.
This approach is useful when researchers need to connect renal pathology with immune or pathogen-related responses. Isolated kidneys can be examined by histology, flow cytometry, immunostaining, culture, or pathogen and immune-response analyses. The resulting data help characterize how infection or experimental disease affects kidney tissue, infiltrating leukocytes, inflammation, and injury within the affected organ.