The Mouse Isolated Perfused Kidney Technique

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

The Mouse Isolated Perfused Kidney Technique

DOI:

10.3791/54712-v

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08:19 min

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November 17, 2016

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Jan Czogalla, Frank Schweda, Johannes Loffing

¹Institute of Anatomy, Swiss National Centre of Competence in Research Kidney,University of Zürich, ²Department of Physiology,University of Regensburg

Chapters

00:05Title
00:45Surgery: Part 1
03:04Surgery: Part 2
06:04Results: Physiology and Morphology of Perfused Kidneys
07:50Conclusion

Summary

Automatic Translation

The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.

The Mouse Isolated Perfused Kidney Technique

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