Wax Imprinter
Wax Imprinter - Related Videos
01:22
Imprinting
0 Views •
2019
Behavioral imprinting is observed in some newborn animals and occurs when they develop strong and specific attachments to another animal (usually a parent) following brief, early-life exposures. Offspring imprint onto parents within a brief period after birth or hatching; this time window is called the critical period. Once imprinting occurs, the bond established between the parents and their offspring is usually long-lasting. Survival Mother sheep imprint onto the scent of their lambs within...
02:30
Genomic Imprinting and Inheritance
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2020
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes. The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
09:23
Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
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Cited by 4 •
2011
Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and determine methylation by bisulfite sequencing.
06:14
Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces
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Cited by 2 •
2018
Presented is an easy method to fabricate nano-micro multiscale structures, for functional surfaces, by aggregating nanofibers fabricated using an anodic aluminum oxide filter.
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
0 Views •
2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
FAQs
Heating melts the wax and allows it to penetrate the porous material, while cooling solidifies the deposited wax. This phase change converts the patterned regions into hydrophobic barriers that remain in place during later fluid handling. The resulting barrier geometry determines how effectively channels or reaction zones stay separated, which is essential for controlled movement during diagnostic assays.
The porous material provides pathways for liquid movement and spaces into which molten wax can penetrate. Once cooled, the wax occupies selected regions and separates neighboring fluid paths without requiring a sealed, nonporous structure. This combination lets a patterned device direct small samples through defined areas while retaining the material’s capacity for capillary fluid movement.
Wax-defined reaction zones localize liquid samples where assay interactions can occur, while surrounding hydrophobic regions limit unintended spreading. This spatial organization supports immunoassays on paper by directing a small sample toward selected test areas rather than across the entire substrate. It also enables multiple regions to be incorporated into a compact platform for immune-marker detection.
Fabrication begins by depositing and patterning wax on the porous material, then applying heat so the wax melts and penetrates selected regions. The material is subsequently cooled, allowing the wax to solidify into hydrophobic boundaries. The finished pattern provides separated channels or reaction zones that can guide liquid samples during a diagnostic test.
Researchers may use this approach when they need a low-cost, portable format for testing small liquid samples for immune markers. Wax-patterned paper can guide the sample through an immunoassay without complex equipment, making the platform suitable for developing point-of-care testing formats. It also supports rapid prototyping when assay layouts or reaction-zone arrangements must be tested.
In infection research, patterned paper devices can provide a platform for detecting infectious agents through guided diagnostic tests. Their portability and limited equipment requirements are relevant when testing formats must operate outside complex laboratory settings. The same fabrication approach allows researchers to rapidly prototype device layouts, helping evaluate compact point-of-care designs for immune-marker or infectious-agent detection.