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Biology

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Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
 

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Article doi: 10.3791/2327
January 28th, 2011

Summary January 28th, 2011

Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and determine methylation by bisulfite sequencing.

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