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09:23 min
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January 28, 2011
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Summary
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Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and determine methylation by bisulfite sequencing.
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Cite this Article
Rea, M., Chen, M., Luan, S., Bhangu, D., Braud, M., Xiao, W. Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm. J. Vis. Exp. (47), e2327, doi:10.3791/2327 (2011).
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