Bounded Sequence

A bounded sequence is a sequence of real or complex numbers whose terms remain within a fixed finite range, making boundedness a fundamental way to control infinite data in analysis. Formally, there is a constant M such that |a_n| ≤ M for every index n; for real sequences, this means the terms have both upper and lower bounds. Boundedness alone does not guarantee convergence, but combined with monotonicity it yields the monotone convergence theorem, while bounded subsequences support the Bolzano–Weierstrass theorem. These results help analyze limits, compactness, approximation, and iterative methods throughout mathematics.

Bounded Sequence - Related Videos

Research

JoVE Journal - Biology

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

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Cited by 6 •

2012

We describe here a technique that is now routinely used to isolate stably bound ribosome nascent chain complexes (RNCs). This technique takes advantage of the discovery that a 17 amino acid long SecM "arrest sequence" can halt translation elongation in a prokaryotic (E. coli) system, when inserted into (or fused to the C-terminus) of virtually any protein.

Isolation of Small Regulatory RNA–Bound Bacterial Target RNA Using Affinity Purification

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2025

Source: Mercier, N., et. al., MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (2021)This video demonstrates the isolation of MS2-tagged small regulatory RNA (sRNA) bound to its bacterial target RNA using affinity purification with a maltose-binding protein (MBP)–MS2 coat protein fusion immobilized on amylose resin.

Amplicon Sequencing using the Long-Read Sequencing Technologies

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2025

This protocol was optimized for targeted deep sequencing of 18 drug-resistance regions in Mycobacterium tuberculosis using a long-read sequencing platform, followed by analysis with a tuberculosis-specific bioinformatics pipeline designed for long-read data.

Research

JoVE Journal - Immunology and Infection
Free Sample

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

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Cited by 8 •

2016

Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.

Screening Inhibitors of Bacterial Membrane-Bound Pyrophosphatase Using a Colorimetric Assay

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2025

Source: Vidilaseris, K., et al. Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors. J. Vis. Exp. (2019)This video demonstrates a colorimetric assay to screen inhibitors of bacterial membrane-bound pyrophosphatase (mPPase) by quantifying orthophosphate release. The method enables identification of compounds that inhibit mPPase from Thermotoga maritima, a potential antimicrobial drug target.

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