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Journal / Immunology and Infection / Flow Cytometric Analysis of Particle-bound Bet v…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Article DOI: 10.3791/54721-v • 10:42 min • November 19th, 2016
November 19th, 2016 •
1, 1, 1, 1
1Environmental Medicine and Health Effects Assessment, Federal Environment Agency

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Summary
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Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.

Tags

Flow Cytometric Analysis Particle-bound Bet V 1 Allergen PM10 Allergen Load Ambient Particulate Matter Particles Flow Cytometry Allergen Visualization Quantification Low Volume Sampler Polytetrafluoroethylene Filters Sample Analysis Freezing Thawing PBS Overlay
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