Adenoviral Gene Delivery
Adenoviral Gene Delivery - Related Videos
07:48
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
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Cited by 9 •
2013
Implantation of cancer cells into the organ of origin can serve as a useful preclinical model to evaluate novel therapies. MB49 bladder carcinoma cells can be grown within the bladder following intravesical instillation. This protocol demonstrates catheterization of the mouse bladder for the purpose of tumor implantation and adenoviral delivery.
03:20
Large-Scale Production of High-Capacity Adenoviral Vectors
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2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the large-scale amplification of high-capacity adenoviral vectors in suspension-adapted human embryonic kidney cells expressing Cre recombinase for use in gene delivery applications requiring high-titer, helper-free vector preparations.
16:59
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
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Cited by 5 •
2012
We describe hepatic neo-islet formation in STZ (streptozotocin)-induced diabetic mice by gene transfer of Neurogenin3 (Ngn3) and Betacellulin (Btc) using helper-dependent adenoviral vector (HDAd) and the reversal of hyperglycemia. Our method takes advantages of helper-dependent adenoviral vectors with their highly efficient in vivo transduction and the long lasting gene expression.
12:30
Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Cited by 12 •
2014
These studies report on reversible attachment of adenoviral gene vectors to coatless metal surfaces of stents and model mesh disks. Sustained release of transduction-competent viral particles contingent upon hydrolysis of cross-linkers used for vector immobilization results in a durable site-specific transgene expression in vascular cells and in stented arteries.
05:00
Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
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Cited by 4 •
2010
This article describes the culture of patient tissue slices for gene delivery studies and subsequent analysis of gene expression using IVIS bioluminescence imaging.
FAQs
Cell-surface receptor binding initiates uptake by endocytosis, after which the vector genome must reach the nucleus to support transgene expression. This route matters because the same delivery strategy can function in both dividing and nondividing cells, broadening the experimental settings in which researchers can examine gene activity or disease-related biology.
Adenoviral gene delivery generally produces strong expression without inserting the transgene into host chromosomes, but that expression is temporary. This profile creates a tradeoff: it can provide a clear short-term effect for experiments or therapeutic development, while limited duration and immune responses must be considered when designing or interpreting studies.
Immune responses are a central design consideration in this method. They can influence how researchers evaluate the strength and duration of transgene expression and how they plan therapeutic or vaccine studies. Consequently, vector design and experimental interpretation must account for both the intended gene effect and the host response.
A basic workflow begins by engineering a recombinant adenoviral vector to carry a selected transgene. Researchers then use the vector with target cells, allowing receptor binding, endocytosis, nuclear release, and subsequent gene expression. In medicine, this sequence supports controlled investigation of gene function and evaluation of candidate therapeutic or vaccine strategies.
Use cases span gene-function studies, vaccine development, cancer research, inherited disorders, and investigations of other diseases. The method is therefore relevant both for asking what a gene does in cells and for exploring whether introducing a selected transgene can support a potential medical approach.
Strong expression can make changes in gene activity easier to examine during a study, whereas temporary expression limits conclusions about long-term effects. For medical research, investigators should distinguish evidence of short-term transgene activity from evidence that a treatment would persist. This distinction is especially relevant when assessing cancer or inherited-disorder applications.