Mesothelial Cell Gaps
Mesothelial Cell Gaps - Related Videos
04:36
Mesothelial Cell Isolation: A Technique to Isolate Primary Mesothelial Cells From Human Omentum Tissue Specimen
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2023
This video describes the protocol to isolate primary human mesothelial cells from the human omentum specimen. The mesothelial cells can be used to establish an in vitro ovarian cancer model to investigate ovarian cancer cell adhesion, invasion, and proliferation.
04:34
Mesothelial Clearance Assay: An In Vitro Technique to Quantify the Invasion Ability of Ovarian Cancer Spheroids into Mesothelial Cell Monolayers
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2023
This video describes a technique to quantitatively measure the interaction of ovarian cancer spheroids with mesothelial cells, using fluorescently labeled cells and time-lapse video microscopy. This in vitro assay models the interaction between metastasizing ovarian cancer cell spheroids and mesothelial cells lining the peritoneal cavity in vivo.
08:54
In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis
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Cited by 47 •
2012
The mesothelial clearance assay described here takes advantage of fluorescently labeled cells and time-lapse video microscopy to visualize and quantitatively measure the interactions of ovarian cancer multicellular spheroids and mesothelial cell monolayers. This assay models the early steps of ovarian cancer metastasis.
14:25
Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
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Cited by 21 •
2014
Ovarian cancer cell invasion into the mesothelial lining of the peritoneum is a dynamic process over time. Utilizing a real time analyzer, the invasive capacity of ovarian cancer cells in a spheroid-mesothelial cell co-culture model can be quantified over prolonged time periods, providing insights into factors regulating the metastatic process.
01:37
Gap Junctions
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2026
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
FAQs
Mesothelial cell gaps arise when cell-cell junctions, cytoskeletal organization, and inflammatory signals alter the cohesion of the mesothelial monolayer. Junctions help maintain contact between neighboring cells, while the cytoskeleton supports their organization. Inflammatory signals can loosen this arrangement, changing barrier continuity and creating discontinuities through which fluid or immune cells may pass.
Gap size provides an indication of how continuous or permeable the mesothelial barrier is. Smaller discontinuities may reflect limited local disruption, whereas larger spaces can permit greater movement of fluid, immune cells, or other materials across the lining. Comparing gap dimensions therefore helps researchers relate structural changes to altered barrier behavior during inflammation or repair.
Tracking both gap formation and closure shows whether the mesothelial layer is becoming more disrupted or recovering continuity. Persistent or enlarging gaps suggest reduced barrier integrity, whereas closure indicates restoration of the monolayer. These changes provide measurable readouts for cell-culture studies examining inflammatory effects, tissue repair, and the mechanisms that regulate barrier stability.
A cell-culture study can follow the appearance, size, and closure of discontinuities within a mesothelial monolayer. Researchers compare these features under conditions that affect junctions, cytoskeletal organization, or inflammatory signaling, then use gap behavior as an indicator of barrier integrity. This approach connects visible structural changes with permeability-related outcomes without requiring the gap itself to be treated as an isolated event.
They are especially relevant when researchers investigate inflammation in pleural or peritoneal tissues and associated fluid accumulation. Disruption of the mesothelial barrier can be studied alongside the movement of fluid and immune cells across the lining. Measuring gap formation helps clarify how inflammatory conditions alter tissue permeability and how restoration of continuity may relate to repair.
Mesothelial cell gaps provide a model for examining how changes in the serous lining may influence the passage of materials, including cancer cells, across a tissue barrier. In medicine, this connects barrier disruption with cancer cell dissemination research. Comparing gap formation, persistence, and closure can help characterize conditions that may support or limit movement through the mesothelial layer.