Epidermal Skin Tissue
Epidermal Skin Tissue - Related Videos
21:16
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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Cited by 57 •
2009
In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical ingredients.
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
06:34
SCAnED - An Open-source Skin Segmentation Macro for Semi-automated Cell and Nuclei Detection in Epidermal and Dermal Skin Compartments
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2025
Here, we present a semi-automated protocol for identifying and quantifying immune and non-immune cells in skin sections using SCAnED, a free ImageJ-based macro for skin segmentation.
01:12
Renewal of Skin Epidermal Stem Cells
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2023
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
10:27
Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
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Cited by 10 •
2011
Dermatitis herpetiformis (DH) is a chronic inflammatory condition characterized by an autoimmune reaction between IgA and epidermal transglutaminase (eTG). DH develops in a very small portion of gluten-sensitive and/or celiac patients. The results of this study indicate that DH can also develop in a rhesus monkey host with symptoms of idiopatic dermatitis.
FAQs
Keratinocytes begin in basal layers and progressively migrate toward the surface, where they produce keratin and organize into lipid-rich layers. This maturation creates a structured barrier rather than a simple cellular covering. The resulting organization helps limit water loss while reducing penetration by physical, chemical, and microbial threats, making keratinocyte development central to epidermal function.
Lipid-rich layers help seal spaces associated with the outward movement of keratinocytes and strengthen the epidermal barrier. Their organization supports control of water loss and contributes to resistance against environmental challenges. In experimental models, changes in this barrier-forming process can therefore provide information about how epidermal tissue maintains protection at the body’s external interface.
Sensory nerve endings located near the epidermis, together with specialized epidermal cells, contribute to the detection and transmission of pain, itch, temperature, and touch-related signals. The epidermis therefore functions as part of a local sensory environment rather than as an isolated barrier. Studying this interface helps connect skin structure with peripheral nervous system activity.
The epidermis provides a tissue context in which skin cells and nearby neural elements can be examined together during neuroimmune communication. This perspective is useful because sensory signaling and local tissue responses may be studied at the same interface. Epidermal research can consequently help investigate interactions relevant to cutaneous sensation, skin disorders, and peripheral nerve function.
Epidermal models allow investigators to examine cutaneous sensation in a controlled tissue system, including responses related to pain, itch, temperature, and touch. When used with tissue-engineered approaches, they can support studies of sensory responses and therapeutic responses. These models also provide a research setting for exploring peripheral nerve function without relying only on intact body tissue.
Tissue-engineered epidermal systems can be applied to investigations of sensory responses and therapeutic responses. Their value lies in providing a constructed tissue context that includes the epidermal features relevant to skin and neural signaling. Such systems may also support research on skin disorders and help examine how candidate interventions relate to the cutaneous sensory environment.
Studying epidermal tissue alongside nearby sensory nerve endings preserves the relationship between the skin interface and peripheral neural signaling. This combined view is important for examining how local tissue structures relate to pain, itch, temperature, and touch. It also supports broader analysis of neuroimmune communication and peripheral nerve function within a cutaneous setting.