Micromanipulator Unit
Micromanipulator Unit - Related Videos
05:45
Micromanipulation of Chromosomes in Insect Spermatocytes
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Cited by 9 •
2018
In this protocol, we describe the selection and preparation of appropriate cells for micromanipulation and the use of a piezoelectric micromanipulator to reposition chromosomes within those cells.
02:10
Single Cell Sampling Using Micromanipulation: A Technique to Isolate Single Cells from Cancer Cell Suspension
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2023
This video describes the technique for isolating single cells from a pool of target cells to carry out downstream analysis. In this method, a micromanipulator fitted to a microscope is used for high-precision isolation of a single target cell.
03:38
Measurement: Standard Units
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2020
Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results. The number in the measurement can be represented in different ways, including decimal form and scientific notation, which is...
03:02
Measurement: Derived Units
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2020
The International System of Units or SI system, by international agreement, has fixed measurement units for seven fundamental properties: length, mass, time, temperature, electric current, amount of substance, and luminosity. These are called the SI base units. Units of measurement derived from the mathematical combination of SI base units are called SI-derived units. For example, the ratio of the SI unit for distance (meter; m) and the SI unit for time (second; s) gives the SI-derived unit...
06:02
Microiontophoresis and Micromanipulation for Intravital Fluorescence Imaging of the Microcirculation
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Cited by 3 •
2011
Microiontophoresis entails movement of ions from a micropipette in response to a difference in electrical potential between the inside and outside of the micropipette. Biologically active molecules are thereby delivered in proportion to electrical current. We illustrate acetylcholine microiontophoresis in conjunction with micromanipulation to study endothelium-dependent vasodilation in the microcirculation.
FAQs
Adjustment controls transmit input through finely geared or motor-driven axes. These axes convert small control changes into incremental movement, allowing an attached electrode, probe, pipette, or other instrument to move forward or backward along a selected direction. The same mechanism also permits the tool to remain at a chosen location during manipulation or measurement.
Mechanical and motorized units both provide controlled positioning through multiple movement axes, but their adjustment systems differ. Mechanical designs transmit movement through geared controls, whereas motorized designs use driven axes. This distinction affects how the operator advances, withdraws, or holds an attached tool, while both approaches support accurate placement when unaided hand motion is insufficient.
Incremental movement limits unintended displacement when an instrument approaches or contacts a selected location. Positional stability then helps keep the electrode, probe, pipette, or tissue interface where it was placed. Together, these features improve manipulation consistency and spatial precision, which are especially important when experiments examine neural structure or function at small physical scales.
The attached instrument determines what the positioning system physically places and maintains. Electrodes can be positioned for recording or stimulation, while probes, pipettes, and other tools support tissue interfaces or cellular manipulation. Because the unit accommodates several instrument types, the same positioning principle can serve different experimental tasks without relying on unaided hand movement.
A basic sequence consists of attaching the selected tool, using the adjustment controls to advance it toward a chosen location, and then withdrawing or holding it as the experiment requires. The controls provide incremental movement along the available axes. This sequence supports deliberate placement of electrodes, probes, pipettes, or other instruments during laboratory manipulation.
They are useful when neuroscience experiments require accurate placement of recording or stimulating electrodes, tissue interfaces, or microinjection tools. Their controlled positioning supports electrophysiology and cellular manipulation, where the location of an instrument can affect the experimental interaction with neural material. They also contribute to reproducible studies of neural structure and function by improving placement consistency.
Precise positioning supports more consistent interactions between an instrument and the selected neural location. In electrophysiology, this can aid the placement of recording or stimulating electrodes; in cellular manipulation, it can support positioning of microinjection tools or other instruments. The resulting control improves spatial precision and helps make observations or manipulations more reproducible across experiments.