Olfactometer Setup
Olfactometer Setup - Related Videos
08:36
Constructing an Olfactometer for Rodent Olfactory Behavior Studies
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Cited by 1 •
2025
This protocol describes the construction of an olfactometer for go/no-go olfactory behavior experiments. Step-by-step instructions, along with images, are provided to ensure the successful construction of the olfactometer. Information for troubleshooting issues encountered during the process is also included.
04:48
Four-Way Olfactometer Assay: A Method to Assess Odorant-Cued Responses in Drosophila
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2023
Fruit flies have a complex olfactory system, can discriminate hundreds of odorants, and use their sense of smell to direct behavioral decisions. This video describes the four-way olfactometer assay, a method to study olfactory behavioral responses in flies. The featured protocol clip shows how to handle test flies and what are critical steps while performing the assay.
08:52
Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer
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Cited by 4 •
2016
We describe here a behavioral setup and data analysis method for assaying olfactory responses of up to 100 vinegar flies (Drosophila melanogaster). This system may be used with single or multiple olfactory stimuli, and adaptable for optogenetic activation or silencing of neuronal subsets.
09:23
A Low Cost Setup for Behavioral Audiometry in Rodents
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Cited by 17 •
2012
A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.
10:13
Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions
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Cited by 12 •
2017
This paper introduces a protocol for the preparation of hemi-larynx specimens facilitating a multi-dimensional view of vocal fold vibration, in order to investigate various biophysical aspects of voice production in humans and non-human mammals.
FAQs
Concentration control depends on mixing odorized air with clean carrier air under regulated airflow. Valves select when the odorized stream enters the delivery path, while tubing carries the mixture toward the subject. Adjusting these coordinated elements allows researchers to present selected concentrations and compare responses to odor intensity under controlled conditions.
Consistent airflow and well-managed valve timing help limit delivery delays, leakage, and cross-contamination between stimuli. These safeguards matter because an organism’s response can depend on both odor identity and the exact time an odor arrives. Reducing unintended carryover makes differences in behavior or neural activity easier to attribute to the intended stimulus.
Calibration helps verify that the system delivers the intended odor conditions consistently, while synchronization aligns odor presentation with behavioral events or neural recordings. Together, these practices improve reproducibility and make stimulus timing more reliable. They also strengthen comparisons across trials when researchers examine how sensory and higher-order brain regions respond.
Researchers can vary odor identity, intensity, and timing, then relate those controlled changes to behavior or brain activity. Odor identity addresses which smell is presented, intensity addresses its selected concentration, and timing supports analysis of responses linked to stimulus onset. This combination helps investigate how olfactory information is represented and processed.
During behavioral experiments, the system presents selected odor stimuli while researchers observe odor-guided responses. During neural recordings, those same controlled presentations provide defined sensory events for relating activity to odor identity, intensity, or timing. This supports comparisons between observable behavior and activity in sensory or higher-order brain regions.
Controlled odor delivery supports investigations of olfactory coding, perception, learning, memory, and odor-guided behavior. Because the system regulates stimulus identity, concentration, and timing, researchers can examine how these features relate to neural activity and behavioral outcomes. The approach is therefore useful for connecting sensory input with processing in broader brain systems.