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Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer
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JoVE Journal Neuroscience
Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer

Olfactory Behaviors Assayed by Computer Tracking Of Drosophila in a Four-quadrant Olfactometer

DOI:
10.3791/54346-v

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08:52 min

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August 20, 2016

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, ,
1The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology,Johns Hopkins University School of Medicine, 2MRC Clinical Sciences Center,Imperial College London

Chapters

  • 00:05Title
  • 00:44Olfactory Stimuli Preparation
  • 01:36Fly Preparation
  • 02:38Behavioral Responses to Attractive and Repellent Odorants
  • 06:02Data Analysis
  • 07:06Results: Measurements and Analysis
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a behavioral setup and data analysis method for assaying olfactory responses of up to 100 vinegar flies (Drosophila melanogaster). This system may be used with single or multiple olfactory stimuli, and adaptable for optogenetic activation or silencing of neuronal subsets.

Tags

Keywords: Olfactory BehaviorsDrosophilaFour-quadrant OlfactometerAutomated Fly TrackingOdorant ChambersOdorant DetectionOlfactory Neural SystemsBehavioral DecisionsOdorant Air StreamsAirflow Rate

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