Pelvic Ganglion Dissection
Pelvic Ganglion Dissection - Related Videos
07:50
Location, Dissection, and Analysis of the Murine Stellate Ganglion
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Cited by 15 •
2020
Pathophysiological changes in the cardiac autonomic nervous system, especially in its sympathetic branch, contribute to the onset and maintenance of ventricular arrhythmias. In the present protocol, we show how to characterize murine stellate ganglia to improve the understanding of the underlying molecular and cellular processes.
09:48
Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats
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Cited by 9 •
2020
The major pelvic ganglia contain parasympathetic and sympathetic neurons that innervate pelvic organs. Here we describe a dissection method and provide schematics for identification of these ganglia and their associated nerves. These methods can be applied to experimental manipulation of these ganglia in vivo or removal post-mortem for further study.
11:08
Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
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Cited by 9 •
2011
We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.
12:42
Pelvic Exam I: Assessment of the External Genitalia
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2023
Source: Alexandra Duncan, GTA, Praxis Clinical, New Haven, CT Tiffany Cook, GTA, Praxis Clinical, New Haven, CT Jaideep S. Talwalkar, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT The pelvic exam can feel invasive to patients, so it is important to do everything possible to make patients feel comfortable and empowered, rather than vulnerable. Clinicians should be aware of how they are communicating, both verbally and nonverbally, and should give their patients...
13:01
Pelvic Exam III: Bimanual and Rectovaginal Exam
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2023
Source: Alexandra Duncan, GTA, Praxis Clinical, New Haven, CT Tiffany Cook, GTA, Praxis Clinical, New Haven, CT Jaideep S. Talwalkar, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT A bimanual exam is a thorough check of a patient's cervix, uterus, and ovaries. It can tell an experienced provider a great deal, as it may lead to the discovery of abnormalities, such as cysts, fibroids, or malignancies. However, it's useful even in the absence of such findings, as it...
FAQs
Anatomical landmarks provide reference points for interpreting the position and course of pelvic ganglia and nerves. Maintaining them helps researchers relate isolated neural structures to nearby pelvic organs rather than viewing each component in isolation. This spatial context supports more reliable analysis of autonomic organization and clarifies how nerve pathways connect with bladder, bowel, or reproductive tissues.
Sequential tracing reveals continuity between ganglia, associated nerves, and their target regions. Instead of examining an isolated structure alone, the investigator follows pathways through the pelvic tissue while removing surrounding material carefully. This approach helps distinguish direct anatomical connections from nearby structures and supports analysis of how peripheral autonomic circuits are organized.
The preparation can show ganglion structure, nerve connections, and relationships between neural elements and pelvic organs. These observations provide anatomical evidence for studying the organization of peripheral autonomic circuits. In neuroscience, that information can be related to signaling, development, or changes in neural arrangement after injury or during disease-associated conditions.
The procedure begins by exposing the pelvic region and removing surrounding tissues carefully. Researchers then identify relevant anatomical landmarks, isolate the ganglia, and trace associated nerves in sequence. Throughout the dissection, preserving recognizable relationships is essential because the final preparation must retain enough spatial information to interpret connections with pelvic organs.
By preserving the relationships between pelvic ganglia, nerves, and organs, this technique provides an anatomical framework for examining autonomic pathways associated with bladder and bowel activity. The resulting preparation can help researchers investigate how these circuits are organized and how their structure may change in studies focused on development, injury, or disease.
Pelvic ganglion dissection supports questions about neuronal organization, signaling pathways, development, and structural responses to injury or disease. Its value comes from linking microscopic or circuit-level observations to recognizable pelvic anatomy. This makes the preparation useful for connecting peripheral autonomic structure with the regulation of reproductive activity as well as visceral function.