Real-time Atp Monitoring
Real-time Atp Monitoring - Related Videos
02:52
Real-Time Monitoring of Odorant Receptor Activation in Recombinant Cells
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2025
Source: de March, C. A. et. al., Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase. J. Vis. Exp. (2019)This video demonstrates the method to monitor the real-time activation of odorant receptors in recombinant mammalian cells using luminescence measurement. Odorant binding triggers G-protein activation and cAMP production, activating glosensor proteins that emit luminescence upon substrate binding, providing a quantitative measure of receptor response.
11:05
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
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Cited by 1 •
2016
Free solution capillary electrophoresis is a fast, cheap and robust analytical method that enables the quantitative monitoring of chemical reactions in real time. Its utility for rapid, convenient and precise analysis is demonstrated here through analysis of covalent peptide grafting onto chitosan films for improved cell adhesion.
03:52
Cellular Impedance-Based Analysis for Monitoring of Cellular Processes in Real-Time
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2025
In this video, we demonstrate the process of real-time monitoring of cellular events, including the proliferation of adherent cells, via a cellular impedance assay. Here, the adherent cells act as insulators, restricting the current flow between the electrodes and increasing the impedance.
04:47
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
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Cited by 7 •
2013
Mammalian cells expressing the bacterial bioluminescence gene cassette (lux) produce light autonomously. The resulting bioluminescent dynamics upon chemical exposure have been demonstrated to reflect the treatment effects on cellular growth and metabolism, making these cells an inexpensive, continuous, real-time toxicity screening tool that can easily be adapted for high-throughput automation.
04:41
Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer
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2025
This video demonstrates the method to analyze energy metabolism in activated human NK cells using an extracellular flux analyzer. Different inhibitors of the electron transport chain complexes impact ATP synthesis, which is measured through the oxygen consumption rate or OCR, providing insights into cellular metabolism and bioenergetics.
FAQs
The sensing domain converts ATP binding into a detectable fluorescent or bioluminescent signal. As ATP concentration changes, the resulting signal changes as well, allowing measurements to follow cellular energy dynamics over time. In neuronal experiments, this mechanism connects molecular ATP availability with activity in living neurons or neural tissues rather than relying only on a single endpoint measurement.
The time course shows how ATP availability changes while neuronal or cellular conditions vary. Researchers can examine ATP fluctuations alongside neuronal activity, synaptic signaling, mitochondrial function, or metabolic stress to determine when energy changes occur relative to neural events. This temporal relationship helps reveal dynamic links between cellular energy status and neural function.
ATP levels can reflect changes associated with neuronal activity, synaptic signaling, mitochondrial function, and metabolic stress. These factors provide distinct biological contexts for interpreting a changing sensor signal. Comparing ATP dynamics under such conditions can help researchers examine how neural work, energy production, and cellular strain are related in neurons or neural tissues.
Both fluorescent and bioluminescent ATP sensors use a sensing domain to produce a measurable signal when ATP binds. Their signal formats differ, but each approach supports observation of ATP changes in living neuronal preparations. The selected format therefore determines how ATP dynamics are recorded, while the biological interpretation remains focused on changing cellular energy availability.
A basic workflow uses a fluorescent or bioluminescent sensor in living neurons or neural tissue and records its signal as ATP changes. Researchers then relate signal fluctuations to neural activity, synaptic signaling, mitochondrial function, or metabolic stress. This approach produces a time-resolved view of energy dynamics that can be connected to neural function.
Researchers can apply real-time ATP monitoring when they need to investigate brain metabolism, neurodegeneration, or injury-related changes in cellular energy. The method is also relevant for studying how drugs or disease-associated mutations affect ATP availability. By observing changes in living neural systems, experiments can connect altered energy dynamics with broader effects on neural cells.
ATP fluctuations can indicate how neural cells respond energetically to disease-associated mutations, drugs, injury, or metabolic stress. Interpreted alongside neural function, these measurements help identify relationships between cellular energy availability and neural performance. The resulting data can support investigation of disease mechanisms, treatment-related cellular effects, and the metabolic consequences of altered neuronal conditions.