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JoVE Journal
Chemistry
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

Full Text
9,541 Views
11:05 min
October 26, 2016

DOI: 10.3791/54549-v

Joel J. Thevarajah1,2,3, Michael D. O'Connor1,4, Patrice Castignolles2,3, Marianne Gaborieau1,2,3

1Molecular Medicine Research Group,Western Sydney University, 2Australian Centre for Research on Separation Science,Western Sydney University, 3School of Science and Health,Western Sydney University, 4School of Medicine,Western Sydney University

Free solution capillary electrophoresis is a fast, cheap and robust analytical method that enables the quantitative monitoring of chemical reactions in real time. Its utility for rapid, convenient and precise analysis is demonstrated here through analysis of covalent peptide grafting onto chitosan films for improved cell adhesion.

The overall goal of this capillary electrophoresis method is to monitor the grafting reaction of peptides onto chitosan films in real time. This method can help answer key questions in the analytical chemistry and polymer chemistry fields, such as how the reaction is proceeding, and how efficient the grafting process is. The main advantage of this technique is that there is no sample preparation needed, and the reaction mixture can be analyzed in real time.

Even though this method can provide insight into reaction processes, it can also be applied towards a system, such as the binding of an anti-cancer drug to a drug carrier, or the digestion of rice or breakfast cereals. Weigh out two grams of glacial acetic acid and complete to 100 milliliters with ultra pure water. Add 100 milliliters of the 2%mass percent acetic acid aqueous solution to 1.7 grams of chitosan powder.

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Capillary ElectrophoresisPeptide GraftingChitosan FilmsReal-time MonitoringAnalytical ChemistryPolymer ChemistryDrug BindingDigestionAcetic AcidCentrifugationFilm PreparationCapillary PreparationFused Silica Capillary

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