Serial Sectioning
Serial Sectioning - Related Videos
09:46
A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
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Cited by 15 •
2018
This study presents reliable and easy procedures for obtaining serial ultrathin sections of a microorganism without expensive equipment in transmission electron microscopy.
10:53
Serial Dilutions and Plating: Microbial Enumeration
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2023
Source: Jonathan F. Blaize1, Elizabeth Suter1, and Christopher P. Corbo1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Quantitative assessment of prokaryotes can be onerous given their abundance, propensity for exponential proliferation, species diversity within a population, and specific physiological needs. Compounding this challenge, is the four-phase nature in which bacteria replicate (lag, log, stationary and death). The ability to accurately...
11:19
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
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Cited by 12 •
2018
This protocol targets specific cells in tissue for imaging at nanoscale resolution using a scanning electron microscope (SEM). Large numbers of serial sections from resin-embedded biological material are first imaged in a light microscope to identify the target and then in a hierarchical manner in the SEM.
01:03
Serial Position Effect
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2025
The serial position effect is a cognitive phenomenon where individuals are more likely to recall the first and last items in a list compared to those in the middle. This effect is divided into the primacy effect and the recency effect. The primacy effect is observed when the initial items in a list are remembered better. This occurs because these items are rehearsed more frequently or receive more elaborative processing, allowing them to be encoded into long-term memory more effectively. For...
01:50
Cross-Sectional Research
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2020
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
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The method preserves the order of adjacent tissue slices so that corresponding structures can be compared from one plane to the next. By aligning these observations, researchers can follow the extent and shape of cells, lesions, fiber tracts, and other anatomical features through the specimen. This sequence provides the basis for two-dimensional reconstruction and three-dimensional mapping.
Section order links observations from separate microscopic fields to their original positions within the specimen. Without that sequence, a feature seen in one slice could not be reliably related to the same feature in neighboring planes. Ordered sections therefore help distinguish a localized structure from one that continues through the tissue and support interpretation of spatial organization.
Serial Sectioning can reveal how cells, lesions, fiber tracts, and other anatomical features extend across successive tissue planes. Examining these features as a sequence helps researchers evaluate their distribution and relationships within preserved nervous tissue. The resulting information is useful for studying neural organization, developmental changes, and disease-related pathology at a three-dimensional scale.
Preserved brain or nervous tissue is first embedded to provide support during cutting. A microtome or cryostat then produces thin sections, which are mounted in their original sequence. The sections can subsequently be stained or labeled for microscopic analysis. Keeping the slices ordered throughout preparation is essential for relating microscopic findings to positions across the specimen.
Both a microtome and a cryostat are used to cut embedded nervous tissue into thin sections, as described for this workflow. Their role is to generate successive slices that can be mounted and examined in sequence. After cutting, staining or labeling makes selected tissue features visible, allowing the ordered series to support anatomical analysis and reconstruction.
The technique is useful when researchers need to examine neural structures across multiple planes rather than in a single section. In neuroscience, it supports investigations of neural organization, developmental changes, disease-related pathology, and relationships between structure and function. Because the sections can be reviewed as an ordered series, the approach also supports two-dimensional reconstruction and three-dimensional mapping.