Vibrating Microtome
Vibrating Microtome - Related Videos
07:28
Measurement of Bioelectric Current with a Vibrating Probe
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Cited by 15 •
2011
The manufacture, calibration and use of non-invasive vibrating probes to measure bioelectric current in various biological systems is described.
01:19
Vibrating Concrete
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2024
Mechanical vibrators are instrumental in compacting newly poured concrete within formwork and around reinforcements. This process is essential to eliminate trapped air pockets and establish a dense concrete mass. One widely used method is vibrating by internal vibrators, often referred to as a poker vibrator or immersion vibrator. It is rapidly inserted through the full depth of the freshly laid concrete and slightly extends into the layer below it (which remains in a plastic state). Consistent...
10:13
Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions
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Cited by 12 •
2017
This paper introduces a protocol for the preparation of hemi-larynx specimens facilitating a multi-dimensional view of vocal fold vibration, in order to investigate various biophysical aspects of voice production in humans and non-human mammals.
07:32
Measurement of Vibration Detection Threshold and Tactile Spatial Acuity in Human Subjects
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Cited by 9 •
2016
Here, we present protocols to determine vibration detection thresholds and tactile acuity using psychophysical methods in man.
01:24
IR Spectroscopy: Molecular Vibration Overview
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2024
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations. Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
FAQs
Rapid blade oscillation reduces the compression and tearing that can occur when a blade moves through soft biological tissue. This mechanical advantage helps produce thinner, more intact sections, which is especially important for delicate brain and spinal cord specimens. Better structural preservation supports clearer microscopic examination and more reliable interpretation of neural organization.
Vibrating Microtome sectioning can process relatively soft tissue without first embedding it in paraffin or freezing it. Avoiding those preparation routes is useful when researchers want to maintain the specimen's existing architecture or work with tissue that is fixed or fresh. The choice therefore supports studies requiring structural preservation and, in suitable preparations, cellular viability.
The instrument can section both fixed and fresh tissue, but the preparation condition relates to the type of information researchers can preserve. Fixed specimens support structural and labeling-based analyses, whereas suitable fresh preparations may retain cellular viability for electrophysiology. This distinction allows sectioning to serve both anatomical investigations and experiments requiring living cellular properties.
A specimen is positioned so that a blade can pass through it while oscillating rapidly, producing a series of thin tissue sections. Brain and spinal cord samples may be fixed or fresh, depending on the intended analysis. Because the approach does not require paraffin embedding or freezing, the resulting sections can be directed toward structural, labeling, or viable-tissue studies.
Researchers use these sections for microscopic examination, immunohistochemistry, electrophysiology, and neuronal tracing. Microscopy reveals tissue organization, immunohistochemistry supports examination through tissue labeling, electrophysiology uses suitable viable preparations, and neuronal tracing helps investigate connectivity. Together, these applications let investigators examine neural structure and function from complementary experimental perspectives.
Vibrating Microtome preparations support investigations of neural organization, connectivity, development, and disease. Preserved architecture helps researchers examine how cells and tissue are arranged, while neuronal tracing addresses connections between regions. Depending on preparation quality and tissue condition, the same general approach can also contribute to studies of cellular function and changes associated with neurological disease.