Xenon Light Source
Xenon Light Source - Related Videos
16:20
Hyperpolarized Xenon for NMR and MRI Applications
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Cited by 28 •
2012
The production of hyperpolarized xenon by means of spin exchange optical pumping (SEOP) is described. This method yields a ~10000-fold enhancement of the nuclear spin polarization of Xe-129 and has applications in nuclear magnetic resonance spectroscopy and imaging. Examples of gas phase and solution state experiments are given.
10:04
Flame Experiments at the Advanced Light Source: New Insights into Soot Formation Processes
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Cited by 4 •
2014
Gas sampling from laboratory-scale flames with online analysis of all species by mass spectrometry is a powerful method to investigate the complex mixture of chemical compounds occurring during combustion processes. Coupled with tunable soft ionization via synchrotron-generated vacuum-ultraviolet radiation, this technique provides isomer-resolved information and potentially fragment-free mass spectra.
10:32
Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
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Cited by 2 •
2021
Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.
06:19
Fixed Target Serial Data Collection at Diamond Light Source
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Cited by 22 •
2021
We present a comprehensive guide to fixed target sample preparation, data collection, and data processing for serial synchrotron crystallography at Diamond beamline I24.
08:35
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
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Cited by 68 •
2021
This paper describes the complete XChem process for crystal-based fragment screening, starting from applying for access and all subsequent steps to data dissemination.
FAQs
Broadband output allows one illumination platform to support experiments that require different wavelengths. By placing optical filters in the light path, researchers can select the spectral portion needed for a fluorescent indicator, structural visualization, or wavelength-dependent stimulation. This flexibility is especially useful when a neuroscience workflow changes optical requirements without changing the illumination source.
Filters act as spectral selectors rather than merely reducing brightness. They transmit chosen wavelengths toward a microscope or fiber-based system, so the delivered light can be matched to the measurement or stimulation requirement. In practice, this separation between source spectrum and selected output helps researchers control which optical signals or responses are produced in neural preparations.
Stable, high-brightness illumination matters because fluorescence measurements and light-based stimulation depend on controlled exposure. Researchers can regulate the conditions under which samples or neural tissue receive light, improving consistency across observations and experiments. The source’s strong output also supports routing light through filters and optical hardware while retaining useful illumination for imaging or stimulation.
A typical setup sends xenon light through a wavelength-selecting filter and then into either a microscope or fiber-based optical path. The chosen route depends on whether the experiment prioritizes imaging or localized light delivery. Researchers then use the configured illumination to excite indicators, visualize structures, or provide wavelength-dependent stimulation under controlled exposure conditions.
In fluorescence microscopy and calcium-imaging workflows, the source supplies selected illumination that enables researchers to observe fluorescent signals associated with neural activity. These measurements can help investigate how neurons behave and how circuits function. Its broadband capability is valuable when different indicators or imaging configurations require different wavelengths within the same general experimental platform.
Optogenetic experiments use the same general optical architecture for a different purpose: delivering light rather than primarily recording fluorescence. Filters define the wavelength sent through the optical system, while fibers can direct that light within the preparation. This enables wavelength-dependent stimulation alongside spatially and temporally controlled investigation of circuit function.