طريقة لقياس حركية الانصهار الفيروسي على مستوى الجسيمات واحدة

Introduction

Preparing Dextran-Functionalized Coverslips

Preparing the Liposomes

Labeling the Virus Particles

Setting Up the Microfluidic Flow Cell

Observing Viral Fusion Kinetics Under the Microscope

Viral Hemifusion and Pore Formation Data: Microscope Footage, Fluorescence Intensity Trajectories, and Lag Times for Viral Hemifusion and Pore Formation

Conclusion