قياس BK-polyomavirus غير الترميز السيطرة المنطقة مدفوعة النشاط النسخي عن طريق قياس التدفق

1:15

Collection of Blood and or Urine Samples and Isolation of BKPyV-DNA

2:11

Amplification and Sequencing of the Non-coding Control Region (NCCR)

3:37

Cloning of the Non-coding Control Region (NCCR) into the Dual Fluorescence Reporter

5:26

Transient Transfection of HEK293T Cells with the Dual Fluorescence Reporter Plasmid and Treatment with Potential Antiviral Agents

7:23

Fluorescence Microscopy and Flow Cytometry

9:22

Results and Data Interpretation

11:26

Conclusion