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07:14 min
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September 20, 2019
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Summary
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هنا نقدم بروتوكول لتقييم ديناميات تشكيل المغزل والتقدم الميتومي. لدينا تطبيق التصوير الفاصل الزمني تمكن المستخدم من تحديد الخلايا في مراحل مختلفة من المنوّد، وتتبع وتحديد العيوب الميتوتيكية، وتحليل ديناميات المغزل ومصير الخلية الميتومية عند التعرض للأدوية المضادة للميثوتيك.
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Cite this Article
Mercadante, D. L., Crowley, E. A., Manning, A. L. Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations. J. Vis. Exp. (151), e60255, doi:10.3791/60255 (2019).
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