نهج قابل للتطوير للغاية لإجراء مسوحات بيئية للديدان الخيطية Caenorhabditis الذاتية

Collection Preparation

Field Collection

Plating Out Field Collections and Isolating Nematodes from Collections

Exporting S-Plates from Fulcrum

Check for Proliferation on S-Plates

Lysis of Isofemale Lines

PCR of SSU and ITS2 Sequences

Identifying Nematodes and Processing the Collection Data

Results: Identifying Nematodes with Sanger Sequencing and Sequence BLAST

Conclusion