تطوير سلالات أنواع الليشمانيا مع التعبير التأسيسي ل eGFP

0:27

Amplification and Insertion of the eGFP Gene into the pLEXSY‐2.1 Vector

2:39

Linearization of the pLEXSY Expression Plasmid

3:26

Transfection of L. panamensis and L. donovani by Electroporation

5:00

Polyclonal Selection in Culture

6:41

Confirmation of Genomic Integration

7:09

Cloning by Limiting Dilution

8:12

Results: Confirmation of eGFP Cassette Expression and Chromosomal Insertion

9:40

Conclusion