إنشاء نظام زراعة مشتركة للعضيات السرطانية المشتقة من المريض في أورام القولون والمستقيم والخلايا الليمفاوية المتسللة للورم (TILs)

Overview

This protocol outlines a method for creating a patient-derived colorectal cancer organoid model co-cultured with tumor-infiltrating lymphocytes (TILs). It aims to study their interactions and the therapeutic potential of TIL-based therapy for personalized colorectal cancer treatment.

Key Study Components

Area of Science

• Colorectal cancer research
• Immunotherapy
• Organoid models

Background

• Colorectal cancer is a leading cause of cancer-related deaths.
• TILs play a crucial role in the immune response against tumors.
• Current therapies often have limited efficacy and high morbidity.
• Patient-derived organoid models can better mimic the tumor microenvironment.

Purpose of Study

• To explore the interactions between TILs and colorectal cancer organoids.
• To evaluate the therapeutic potential of TIL-based therapies.
• To develop a preclinical platform for personalized treatment strategies.

Methods Used

• Preparation of experimental reagents and lab wear.
• Minced colorectal cancer tissue is digested and dissociated.
• Co-culture of TILs with organoids to study interactions.
• Quantification of viable cells using trypan blue.

Main Results

• The protocol successfully isolates TILs for co-culture.
• Interactions between TILs and organoids can be studied effectively.
• The model provides insights into immune responses in colorectal cancer.
• Potential for predicting efficacy of TIL-based therapies.

Conclusions

• This organoid model is a valuable tool for colorectal cancer research.
• It enhances understanding of TIL interactions in the tumor microenvironment.
• The findings may lead to improved personalized treatment options.

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