Karen M. Polizzi

Centre for Synthetic Biology and Innovation, Imperial College London

Research Area

Biography

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JoVE Journal Publications

ArticleTotal : 2	Year
Publication title	2014
Publication title Cited by 1	2022

Other Publications

Article	Year
Simulation modeling of pooling for combinatorial protein engineering. Journal of biomolecular screening\| PubMed ID: 16234344	2005
Pooling for improved screening of combinatorial libraries for directed evolution. Biotechnology progress\| PubMed ID: 16889370	2006
Structure-guided consensus approach to create a more thermostable penicillin G acylase. Biotechnology journal\| PubMed ID: 16892288	2006
High-throughput screening for enhanced protein stability. Current opinion in biotechnology\| PubMed ID: 17049838	2006
Better library design: data-driven protein engineering. Biotechnology journal\| PubMed ID: 17183506	2007
Stability of biocatalysts. Current opinion in chemical biology\| PubMed ID: 17307381	2007
Revealing biases inherent in recombination protocols. BMC biotechnology\| PubMed ID: 18001472	2007
Metabolic network reconstruction: advances in in silico interpretation of analytical information. Current opinion in biotechnology\| PubMed ID: 22119273	2012
Genome-based kinetic modeling of cytosolic glucose metabolism in industrially relevant cell lines: Saccharomyces cerevisiae and Chinese hamster ovary cells. Bioprocess and biosystems engineering\| PubMed ID: 22286123	2012
The role of ER stress-induced apoptosis in neurodegeneration. Current Alzheimer research\| PubMed ID: 22299619	2012
Protein construct optimization: data sharing strategy. Protein & cell\| PubMed ID: 22410785	2012
Binding of human BiP to the ER stress transducers IRE1 and PERK requires ATP. Biochemical and biophysical research communications\| PubMed ID: 22446326	2012
In situ monitoring of intracellular glucose and glutamine in CHO cell culture. PloS one\| PubMed ID: 22509313	2012
What is synthetic biology? Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 23996434	2013
An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells. Analytical biochemistry\| PubMed ID: 24036437	2013
Comparative analysis of amino acid metabolism and transport in CHO variants with different levels of productivity. Journal of biotechnology\| PubMed ID: 24056080	2013
Opportunities for bioprocess monitoring using FRET biosensors. Biochemical Society transactions\| PubMed ID: 24059500	2013
Can too many copies spoil the broth? Microbial cell factories\| PubMed ID: 24354594	2013
Towards controlling the glycoform: a model framework linking extracellular metabolites to antibody glycosylation. International journal of molecular sciences\| PubMed ID: 24637934	2014
Co-culture systems and technologies: taking synthetic biology to the next level. Journal of the Royal Society, Interface\| PubMed ID: 24829281	2014
Genetically-encoded biosensors for monitoring cellular stress in bioprocessing. Current opinion in biotechnology\| PubMed ID: 25151059	2015
How does mild hypothermia affect monoclonal antibody glycosylation? Biotechnology and bioengineering\| PubMed ID: 25545631	2015
Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris. Microbial cell factories\| PubMed ID: 26849882	2016
A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation. Scientific reports\| PubMed ID: 27345611	2016
Model-based investigation of intracellular processes determining antibody Fc-glycosylation under mild hypothermia. Biotechnology and bioengineering\| PubMed ID: 27869292	2017
Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production. Biotechnology and bioengineering\| PubMed ID: 28112405	2017
Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris. AMB Express\| PubMed ID: 28342171	2017
Sense and sensitivity in bioprocessing-detecting cellular metabolites with biosensors. Current opinion in chemical biology\| PubMed ID: 28609710	2017
Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation. Biotechnology and bioengineering\| PubMed ID: 28921534	2018
A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Scientific reports\| PubMed ID: 29150665	2017
A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization. Protein expression and purification\| PubMed ID: 29601964	2018
Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 29666238	2018
Computational Re-design of Synthetic Genetic Oscillators for Independent Amplitude and Frequency Modulation. Cell systems\| PubMed ID: 29680377	2018
Investigating the consequences of asymmetric endoplasmic reticulum inheritance in under stress using a combination of single cell measurements and mathematical modelling. Synthetic and systems biotechnology\| PubMed ID: 29911200	2018
Tools for engineering coordinated system behaviour in synthetic microbial consortia. Nature communications\| PubMed ID: 29992956	2018
Biosensor-assisted engineering of a high-yield Pichia pastoris cell-free protein synthesis platform. Biotechnology and bioengineering\| PubMed ID: 30552674	2019
Whole-Cell Biosensor with Tunable Limit of Detection Enables Low-Cost Agglutination Assays for Medical Diagnostic Applications. ACS sensors\| PubMed ID: 30623662	2019
Low-cost and user-friendly biosensor to test the integrity of mRNA molecules suitable for field applications. Biosensors & bioelectronics\| PubMed ID: 31100599	2019
BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms. Microbial biotechnology\| PubMed ID: 31328393	2020
A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure. Scientific reports\| PubMed ID: 31462650	2019
Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Cell-Free System. Frontiers in bioengineering and biotechnology\| PubMed ID: 32117947	2020
Improving the reaction mix of a cell-free system using a design of experiments approach to minimise experimental effort. Synthetic and systems biotechnology\| PubMed ID: 32637667	2020
Microbial interventions are an easier alternative to engineer higher organisms. Microbial biotechnology\| PubMed ID: 33070407	2021
Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System. Current protocols in protein science\| PubMed ID: 33108045	2020
Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris. Traffic (Copenhagen, Denmark)\| PubMed ID: 33263222	2021
Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform. Frontiers in bioengineering and biotechnology\| PubMed ID: 33604330	2020
Refactoring of a synthetic raspberry ketone pathway with EcoFlex. Microbial cell factories\| PubMed ID: 34112158	2021
Exploring conformational preferences of proteins: ionic liquid effects on the energy landscape of avidin. Chemical science\| PubMed ID: 34163590	2020
An experimental approach probing the conformational transitions and energy landscape of antibodies: a glimmer of hope for reviving lost therapeutic candidates using ionic liquid. Chemical science\| PubMed ID: 34349928	2021
Correction to: Refactoring of a synthetic raspberry ketone pathway with EcoFlex. Microbial cell factories\| PubMed ID: 34407814	2021
Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics. Current opinion in biotechnology\| PubMed ID: 34438131	2021
Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 34611868	2022
GenoChemetic Strategy for Derivatization of the Violacein Natural Product Scaffold. ACS chemical biology\| PubMed ID: 34648268	2021
High-yield 'one-pot' biosynthesis of raspberry ketone, a high-value fine chemical. Synthetic biology (Oxford, England)\| PubMed ID: 34712844	2021
Expression of proteins in Pichia pastoris. Methods in enzymology\| PubMed ID: 34742398	2021
Cell-free protein synthesis using Chinese hamster ovary cells. Methods in enzymology\| PubMed ID: 34752298	2021
Model Identifies Genetic Predisposition of Alzheimer's Disease as Key Decider in Cell Susceptibility to Stress. International journal of molecular sciences\| PubMed ID: 34769426	2021
Production and purification of an active CRM197 in Pichia pastoris and its immunological characterization using a Vi-typhoid antigen vaccine. Vaccine\| PubMed ID: 34774362	2021
Cell-Free Protein Synthesis Using Pichia pastoris. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 34985738	2022